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CD71/Transferrin Receptor Rabbit pAb (A5865)

Publications (73) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CD71/Transferrin Receptor Rabbit pAb (A5865)

Western blot analysis of lysates from HeLa cells using CD71/Transferrin Receptor Rabbit pAb (A5865) at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using CD71/Transferrin Receptor Rabbit pAb (A5865) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD71/Transferrin Receptor Rabbit pAb (A5865) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using CD71/Transferrin Receptor Rabbit pAb (A5865) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry analysis of paraffin-embedded Human placenta using CD71/Transferrin Receptor Rabbit pAb (A5865) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunofluorescence analysis of PC-12 cells using CD71/Transferrin Receptor Rabbit pAb (A5865) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCD71/Transferrin Receptor Rabbit pAb
Catalog No.A5865
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human CD71/Transferrin Receptor (NP_001121620.1).
SequenceMMDQARSAFSNLFGGEPLSYTRFSLARQVDGDNSHVEMKLAVDEEENADNNTKANVTKPKRCSGSICYGTIAVIVFFLIGFMIGYLGYCKGVEPKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDF
Gene ID
Swiss Prot
SynonymsT9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46; CD71/Transferrin Receptor
Calculated MW85kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1500 - 1:9000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationCell membrane, Melanosome, Secreted, Single-pass type II membrane protein.
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus, Mus musculus, Danio rerio, Capra hircus)

IF(Rattus norvegicus, Capra hircus, Mus musculus, Gallus gallus)

IHC(Mus musculus, Homo sapiens)

IF(Homo sapiens, Rattus norvegicus, Mus musculus)

IHC(Homo sapiens, Mus musculus)

Co-IP(Mus musculus)

IF(Mus musculus)

IHC(Mus musculus)

WB(Mus musculus)

Other(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - CD71/Transferrin Receptor Rabbit pAb (A5865)}

Western blot - CD71/Transferrin Receptor Rabbit pAb (A5865)

Western blot analysis of lysates from HeLa cells using CD71/Transferrin Receptor Rabbit pAb (A5865) at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)}

Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using CD71/Transferrin Receptor Rabbit pAb (A5865) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)}

Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD71/Transferrin Receptor Rabbit pAb (A5865) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)}

Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using CD71/Transferrin Receptor Rabbit pAb (A5865) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)}

Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry analysis of paraffin-embedded Human placenta using CD71/Transferrin Receptor Rabbit pAb (A5865) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - CD71/Transferrin Receptor Rabbit pAb (A5865)}

Immunofluorescence - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunofluorescence analysis of PC-12 cells using CD71/Transferrin Receptor Rabbit pAb (A5865) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A5865? Please let us know so that we can cite the reference in this datasheet.

항체 (4)

ABflo® 488 Rabbit anti-Human CD71 mAb
A22301

FC

Reactivity:Human

ABflo® 488 Rabbit anti-Human CD71 mAb

CD71/Transferrin Receptor Rabbit mAb
A22161

WBIHC-PIF/ICCELISA

Reactivity:Human

CD71/Transferrin Receptor Rabbit mAb

CD71/Transferrin Receptor Rabbit pAb
A21622

WBIF/ICCIPELISA

Reactivity:Human,Mouse,Rat

CD71/Transferrin Receptor Rabbit pAb

ABflo® 594 Rabbit anti-Human CD71 mAb
A24257

FC

Reactivity:Human

ABflo® 594 Rabbit anti-Human CD71 mAb

재조합 단백질 (3)

Recombinant Human sTfR/CD71 Protein
RP00085

Tags:C-His

Expressed Host:HEK293 cells

Recombinant Human sTfR/CD71 Protein

Recombinant Human sTfR/CD71 Protein
RP00371

Tags:C-hFc

Expressed Host:HEK293 cells

Recombinant Human sTfR/CD71 Protein

Biotinylated Recombinant Human sTfR/CD71 Protein
RP00372B

Tags:N-His&Avi

Expressed Host:HEK293 cells

Biotinylated Recombinant Human sTfR/CD71 Protein

ELISA 키트 (1)

Human Transferrin receptor (TFR) ELISA Kit
RK00342

Human Transferrin receptor (TFR) ELISA Kit

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)