제품 > 항체 > 단일클론항체(mAb)

CD80 Rabbit mAb (A25138)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates using CD80 Rabbit mAb (A25138) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): 293F.
Exposure time: 90s.

ABclonal:Flow CytoMetry - CD80 Rabbit mAb (A25138)

Flow cytometry: 1X10^6 293F cells (negative control , left) and Raji cells (right) were surface-stained with CD80 Rabbit mAb (A25138, 2 μg/mL, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line), followed by ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Raji cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or CD80 Rabbit mAb (A25138, 2 μg/mL, right).

Overview

Product name	CD80 Rabbit mAb
Catalog No.	A25138
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61136

Background

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 35-242 of human CD80 (NP_005182.1).
Sequence	VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDN
Gene ID
Swiss Prot
Synonyms	CD80; B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7; CD80 molecule
Calculated MW	18kDa/29kDa/33kDa
Observed MW	50-75kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 FC 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Flow Cytometry
Positive samples	Raji
Cellular location	Cell surface, external side of plasma membrane, plasma membrane

Documents

Certificate of Compliance

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Research Area

CD80 Rabbit mAb images

Western blot - CD80 Rabbit mAb (A25138)

Flow CytoMetry - CD80 Rabbit mAb (A25138)

Flow cytometry: 1X10^6 Raji cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or CD80 Rabbit mAb (A25138, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.