제품 > 항체 > 다중클론항체(pAb)

CD81 Rabbit pAb (A5270)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Rat brain, using CD81 Rabbit pAb (A5270) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of various lysates, using CD81 Rabbit pAb (A5270) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): Hep G2
Exposure time: 20s.

ABclonal:Immunofluorescence - CD81 Rabbit pAb (A5270)

Immunofluorescence analysis of THP-1 and U-937 cells using CD81 Rabbit pAb (A5270) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	CD81 Rabbit pAb
Catalog No.	A5270
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 114-199 of human CD81 (NP_004347.1).
Sequence	VNKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFS
Gene ID
Swiss Prot
Synonyms	S5.7; CVID6; TAPA1; TSPAN28; CD81
Calculated MW	26kDa
Observed MW	22kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Raji, Mouse brain
Cellular location	Membrane, Multi-pass membrane protein.
Customer validation	(HeLa cells) WB(Homo sapiens, Gallus gallus, Rattus norvegicus, Mus musculus, Cynoglossus semilaevis, Sus scrofa, Arabidopsis thaliana) IF(Homo sapiens,Mus musculus, Homo sapiens, Rattus norvegicus) WB(Homo sapiens,Mus musculus) IF(Mus musculus) IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

연구면역학 & 염증CDs

CD81 Rabbit pAb images

Western blot - CD81 Rabbit pAb (A5270)

Immunofluorescence - CD81 Rabbit pAb (A5270)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Theranostics

Application:

IF:

12.4

Species:

Homo sapiens

PMID:

31149048

Title:

Localized injection of miRNA-21-enriched extracellular vesicles effectively restores cardiac function after myocardial infarction.

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Reprod Domest Anim

Application:

IF:

1.6

Species:

Gallus gallus

PMID:

34717018

Title:

Characterization and proteomics of chicken seminal plasma extracellular vesicles

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Tissue Cell

Application:

IF:

2.7

Species:

Rattus norvegicus

PMID:

34823099

Title:

Bone marrow mesenchymal stem cell derived exosomal miR-455-5p protects against spinal cord ischemia reperfusion injury

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Journal of cellular and molecular medicine

Application:

IF:

5.3

Species:

Mus musculus

PMID:

34953015

Title:

Gingival mesenchymal stem cell‐derived exosomes are immunosuppressive in preventing collagen‐induced arthritis

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Frontiers in immunology

Application:

IF:

5.7

Species:

Cynoglossus semilaevis

PMID:

36059498

Title:

Exosomal miRNAs in the plasma of Cynoglossus semilaevis infected with Vibrio harveyi: Pleiotropic regulators and potential biomarkers involved in inflammatory and immune responses - PMC

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Rattus norvegicus

PMID:

36516531

Title:

Huc-MSCs-derived exosomes attenuate neuropathic pain by inhibiting activation of the TLR2/MyD88/NF-κB signaling pathway in the spinal microglia by …

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Cells

Application:

IF:

7.7

Species:

Mus musculus

PMID:

36552720

Title:

Transcriptome Analysis Unveils That Exosomes Derived from M1-Polarized Microglia Induce Ferroptosis of Neuronal Cells - PMC

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Calcified tissue international

Application:

IF:

3.3

Species:

Rattus norvegicus

PMID:

36840756

Title:

1α, 25-Dihydroxyvitamin D3 Regulates microRNA Packaging in Extracellular Matrix Vesicles and Their Release in the Matrix

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Open medicine (Warsaw, Poland)

Application:

IF:

Species:

Homo sapiens

PMID:

36941991

Title:

Exosomal miR-93-5p regulated the progression of osteoarthritis by targeting ADAMTS9 - PMC

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Frontiers in immunology

Application:

IF:

5.7

Species:

Homo sapiens

PMID:

37153544

Title:

Endothelial ACKR1 is induced by neutrophil contact and down-regulated by secretion in extracellular vesicles - PMC

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Molecular and cellular biochemistry

Application:

IF:

3.5

Species:

Homo sapiens

PMID:

37812348

Title:

Exosome miRNA-203 promotes M1 macrophage polarization and inhibits prostate cancer tumor progression

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Nature communications

Application:

IF:

16.6

Species:

Mus musculus

PMID:

38253527

Title:

EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses - PMC

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

International journal of nanomedicine

Application:

IF, WB

IF:

6.6

Species:

Homo sapiens,Mus musculus

PMID:

38567379

Title:

Exosomes Derived from Human Palatal Mesenchymal Cells Mediate Intercellular Communication During Palatal Fusion by Promoting Oral Epithelial Cell Migration

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

J Exp Clin Cancer Res

Application:

WB, IF

IF:

Species:

Mus musculus

PMID:

34088337

Title:

LINC00511 drives invasive behavior in hepatocellular carcinoma by regulating exosome secretion and invadopodia formation

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Veterinary microbiology

Application:

IF:

2.4

Species:

Sus scrofa

PMID:

38518631

Title:

Exosomes Secreted by CSFV-Infected Cells Evade Neutralizing Antibody to Activate Innate Immune Responses and Establish Productive Infection in Recipient Cells

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Journal of tissue engineering

Application:

IF:

6.7

Species:

Homo sapiens,Rattus norvegicus

PMID:

39157647

Title:

EVs from cells at the early stages of chondrogenesis delivered by injectable SIS dECM promote cartilage regeneration - PMC

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

BMC gastroenterology

Application:

IHC

IF:

2.8

Species:

Homo sapiens

PMID:

39179979

Title:

RARB associated with MSI, affects progression and prognosis of gastric cancer - PMC

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Andrology

Application:

IF:

4.6

Species:

Homo sapiens

PMID:

28914500

Title:

Comprehensive proteomics analysis of exosomes derived from human seminal plasma.

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Leuk Res

Application:

IF:

2.1

Species:

Mus musculus

PMID:

34875555

Title:

MiR-665 suppresses the progression of diffuse large B cell lymphoma (DLBCL) through targeting LIM and SH3 protein 1 (LASP1)

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Aging

Application:

IF:

3.9

Species:

Homo sapiens

PMID:

39213192

Title:

Cigarette smoke-induced exosomal miR-221-3p facilitates M1 macrophage polarization via the STAT3 pathway in chronic obstructive pulmonary disease - PMC

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Bioactive materials

Application:

IF:

18.9

Species:

Arabidopsis thaliana

PMID:

39247401

Title:

Bioprinted mesenchymal stem cell microfiber-derived extracellular vesicles alleviate unilateral renal ischemia-reperfusion injury and fibrosis by inhibiting tubular epithelial cells ferroptosis - PMC

References for CD81 Rabbit pAb (A5270)

Product:

CD81 Rabbit pAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Mus musculus

PMID:

39407245