제품 > 항체 > 다중클론항체(pAb)

CD86 Rabbit pAb (A1199)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates using CD86 Rabbit pAb (A1199) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): Jurkat
Exposure time: 90s.

ABclonal:Immunofluorescence - CD86 Rabbit pAb (A1199)

Immunofluorescence analysis of Raji cells using CD86 Rabbit pAb (A1199) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	CD86 Rabbit pAb
Catalog No.	A1199
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 25-133 of human CD86 (NP_787058.4).
Sequence	PLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVL
Gene ID
Swiss Prot
Synonyms	B70; B7-2; B7.2; LAB72; CD28LG2; CD86
Calculated MW	38kDa
Observed MW	60-85kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Raji
Cellular location	Cell membrane, Single-pass type I membrane protein.
Customer validation	(Human cells) WB(Mus musculus, Homo sapiens, Rattus norvegicus) IF(Rattus norvegicus, Mus musculus, Homo sapiens, Oryctolagus cuniculus) IHC(Mus musculus, Rattus norvegicus, Homo sapiens) IP(Homo sapiens) IF(Homo sapiens, Mus musculus, Rattus norvegicus) FC(Mus musculus) IHC(Rattus norvegicus) Co-IP(Homo sapiens,Mus musculus) IF(Homo sapiens,Mus musculus) IHC(Homo sapiens,Mus musculus) IP(Homo sapiens,Mus musculus) WB(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CD86 Rabbit pAb images

Western blot - CD86 Rabbit pAb (A1199)

Immunofluorescence - CD86 Rabbit pAb (A1199)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A1199

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Product Protocols

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

American Journal of Reproductive Immunology

Application:

IF:

3.886

Species:

Mus musculus

PMID:

28054406

Title:

Surfactant protein D regulates murine testicular immune milieu and sperm functions

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Stem Cells and Development

Application:

IF:

3.272

Species:

Mus musculus

PMID:

31918626

Title:

RNA-Seq Reveals Underlying Transcriptomic Mechanisms of Bone Marrow-Derived Mesenchymal Stem Cells in the Regulation of Microglia-Mediated Neuroinflammation After Subarachnoid Hemorrhage

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Sci China Life Sci

Application:

IF:

6.038

Species:

Mus musculus

PMID:

34125371

Title:

Punicalagin ameliorates collagen-induced arthritis by downregulating M1 macrophage and pyroptosis via NF-κB signaling pathway

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Acta Biomater

Application:

IF:

8.947

Species:

Rattus norvegicus

PMID:

34245890

Title:

Soft apoptotic-cell-inspired nanoparticles persistently bind to macrophage membranes and promote anti-inflammatory and pro-healing effects

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

3 Biotech

Application:

IF:

Species:

Rattus norvegicus

PMID:

34881164

Title:

Tyrosine kinase nonreceptor 1 (TNK1) knockdown ameliorates hemorrhage shock-induced kidney injury via inhibiting macrophage M1 polarization

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Genes to cells : devoted to molecular & cellular mechanisms

Application:

IF:

1.89

Species:

Mus musculus

PMID:

34228857

Title:

MiR-382-5p suppresses M1 macrophage polarization and inflammatory response in response to bronchopulmonary dysplasia through targeting CDK8: Involving inhibition of STAT1 pathway

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Translational oncology

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

36191460

Title:

Development and validation of a microenvironment-related prognostic model for hepatocellular carcinoma patients based on histone deacetylase family - PMC

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Biochemical pharmacology

Application:

IHC

IF:

6.1

Species:

Mus musculus

PMID:

35381211

Title:

Tuftsin ameliorates splenic inflammatory injury by promoting neuropilin-1 in severe acute pancreatitis

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Neurochemistry international

Application:

IF:

4.29

Species:

Rattus norvegicus

PMID:

35429577

Title:

DJ-1 activates the Atg5-Atg12-Atg16L1 complex via Sirt1 to influence microglial polarization and alleviate cerebral ischemia/reperfusion-induced inflammatory injury

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Journal of controlled release : official journal of the Controlled Release Society

Application:

IF:

11.46

Species:

Rattus norvegicus

PMID:

35489544

Title:

Opsonized nanoparticles target and regulate macrophage polarization for osteoarthritis therapy: A trapping strategy

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

7.41

Species:

Rattus norvegicus

PMID:

35486975

Title:

Dexmedetomidine attenuates perioperative neurocognitive disorders by suppressing hippocampal neuroinflammation and HMGB1/RAGE/NF-κB signaling pathway

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IHC

IF:

7.41

Species:

Mus musculus

PMID:

36516692

Title:

Avicularin alleviates osteoporosis-induced implant loosening by attenuating macrophage M1 polarization via its inhibitory effect on the activation of NF-κB

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Biomaterials research

Application:

IHC

IF:

15.86

Species:

Rattus norvegicus

PMID:

36755333

Title:

Bioactive self-healing hydrogel based on tannic acid modified gold nano-crosslinker as an injectable brain implant for treating Parkinsons disease - PMC

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

ACS applied materials & interfaces

Application:

IF:

10.38

Species:

Homo sapiens

PMID:

36562739

Title:

Acceleration of Oral Wound Healing under Diabetes Mellitus Conditions Using Bioadhesive Hydrogel

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Journal of immunology (Baltimore, Md. : 1950)

Application:

IHC

IF:

5.42

Species:

Homo sapiens

PMID:

35649629

Title:

The IL-33/ST2 Axis Promotes Primary Sjögren's Syndrome by Enhancing Salivary Epithelial Cell Activation and Type 1 Immune Response

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Frontiers in medicine

Application:

IF:

5.05

Species:

Mus musculus

PMID:

36714100

Title:

Cryptotanshinone attenuates LPS-induced acute lung injury by regulating metabolic reprogramming of macrophage - PMC

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Inflammation research : official journal of the European Histamine Research Society ... [et al.]

Application:

IF:

6.98

Species:

Mus musculus

PMID:

36912917

Title:

Activating α7nAChR helps post-myocardial infarction healing by regulating macrophage polarization via the STAT3 signaling pathway

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi

Application:

IHC

IF:

10.27

Species:

Mus musculus

PMID:

36964051

Title:

Salmonella typhimurium exacerbates injuries but resolves fibrosis in liver and spleen during Schistosoma mansoni infection

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Mus musculus

PMID:

36786689

Title:

(-)-Epicatechin gallate blocked cellular foam formation in atherosclerosis by modulating CD36 expression in vitro and in vivo

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Heliyon

Application:

IF:

3.77

Species:

Mus musculus

PMID:

37153438

Title:

BushenHuoxue decoction suppresses M1 macrophage polarization and prevents LPS induced inflammatory bone loss by activating AMPK pathway - PMC

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Heliyon

Application:

IF:

3.77

Species:

Mus musculus

PMID:

37153438

Title:

BushenHuoxue decoction suppresses M1 macrophage polarization and prevents LPS induced inflammatory bone loss by activating AMPK pathway - PMC

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Cells

Application:

IHC

IF:

7.66

Species:

Rattus norvegicus

PMID:

37048067

Title:

Modeling the Effects of Cypermethrin Toxicity on Ovalbumin-Induced Allergic Pneumonitis Rats: Macrophage Phenotype Differentiation and p38/STAT6 Signaling Are Candidate Targets of Pirfenidone Treatment - PMC

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Life sciences

Application:

IF:

6.78

Species:

Homo sapiens

PMID:

37295712

Title:

A fluorene derivative inhibits human hepatocellular carcinoma cells by ROS-mediated apoptosis, anoikis and autophagy

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.19

Species:

Rattus norvegicus

PMID:

37480967

Title:

Water extracts of Tibetan medicine Wuweiganlu attenuates experimental arthritis via inducing macrophage polarization towards the M2 type

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Cell proliferation

Application:

IF:

8.75

Species:

Oryctolagus cuniculus

PMID:

37723928

Title:

Promotion of diced cartilage survival and regeneration with grafting of small intestinal submucosa loaded with urinederived stem cells

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Cell death & disease

Application:

WB, IP

IF:

Species:

Homo sapiens

PMID:

36639372

Title:

Glioma-derived LRIG3 interacts with NETO2 in tumor-associated macrophages to modulate microenvironment and suppress tumor growth - PMC

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

International journal of biological sciences

Application:

WB, IF

IF:

10.75

Species:

Homo sapiens

PMID:

35002512

Title:

Trophoblast-derived Lactic Acid Orchestrates Decidual Macrophage Differentiation via SRC/LDHA Signaling in Early Pregnancy

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Advanced healthcare materials

Application:

IF:

11.09

Species:

Homo sapiens,Rattus norvegicus

PMID:

37949452

Title:

Wireless Electric Cues Mediate Autologous DPSCLoaded Conductive Hydrogel Microspheres to Engineer The ImmunoAngiogenic Niche for Homologous

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Carbohydrate polymers

Application:

IF:

10.72

Species:

Rattus norvegicus

PMID:

38142066

Title:

A guanosine/konjac glucomannan supramolecular hydrogel with antioxidant, antibacterial and immunoregulatory properties for cutaneous wound treatment

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Redox biology

Application:

IF:

10.78

Species:

Rattus norvegicus

PMID:

38241836

Title:

Endothelial transferrin receptor 1 contributes to thrombogenesis through cascade ferroptosis - PMC

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Cellular and molecular neurobiology

Application:

IF:

4.23

Species:

Rattus norvegicus

PMID:

38363424

Title:

Expression of CGRP in the Trigeminal Ganglion and Its Effect on the Polarization of Macrophages in Rats with Temporomandibular Arthritis - PMC

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Cancer letters

Application:

IF:

9.75

Species:

Homo sapiens

PMID:

38360142

Title:

Acidosis activates breast cancer ferroptosis through ZFAND5/SLC3A2 signaling axis and elicits M1 macrophage polarization

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Journal of neurochemistry

Application:

IF:

4.7

Species:

Rattus norvegicus

PMID:

38430009

Title:

Unmoving and uninflamed: Characterizing neuroinflammatory dysfunction in the WistarKyoto rat model of depression

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

15.1

Species:

Mus musculus

PMID:

38342630

Title:

Inflammatory FibroblastLike SynoviocyteDerived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Cell Death Dis

Application:

IF:

9.685

Species:

Mus musculus

PMID:

33093453

Title:

Exosomal LGALS9 in the cerebrospinal fluid of glioblastoma patients suppressed dendritic cell antigen presentation and cytotoxic T-cell immunity

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Acta pharmaceutica Sinica. B

Application:

IF:

14.5

Species:

Mus musculus

PMID:

38486995

Title:

Biomimetic "gemini nanoimmunoregulators" orchestrated for boosted photoimmunotherapy by spatiotemporally modulating PD-L1 and tumor-associated

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Brain Research Bulletin

Application:

WB, IHC

IF:

3.715

Species:

Rattus norvegicus

PMID:

32092434

Title:

Oral treatment with glycyrrhizin inhibits NLRP3 inflammasome activation and promotes microglial M2 polarization after traumatic spinal cord injury

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

WB, IF

IF:

5.19

Species:

Mus musculus

PMID:

37263314

Title:

Modified Xiaoyao San reverses lipopolysaccharide-induced depression-like behavior through suppressing microglia M1 polarization via enhancing autophagy

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Journal of inflammation research

Application:

WB, IF

IF:

4.63

Species:

Mus musculus

PMID:

35668915

Title:

Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway - PMC

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Immunopharmacol

Application:

IF:

5.714

Species:

Mus musculus

PMID:

33744777

Title:

Loganin alleviates sepsis-induced acute lung injury by regulating macrophage polarization and inhibiting NLRP3 inflammasome activation

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Asian journal of pharmaceutical sciences

Application:

IF:

9.27

Species:

Mus musculus

PMID:

36101893

Title:

Non-cytotoxic Nanoparticles Re-educating Macrophages Achieving both Innate and Adaptive Immune Responses for Tumor Therapy

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Advanced healthcare materials

Application:

IF:

11.09

Species:

Mus musculus

PMID:

35543500

Title:

Low Surface Accessible Area NanoCoral TiO 2 for the Reduction of Foreign Body Reaction During Implantation

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Pharmaceutical biology

Application:

WB, IF

IF:

3.88

Species:

Rattus norvegicus

PMID:

36239618

Title:

Shenlian extract attenuates myocardial ischaemia-reperfusion injury via inhibiting M1 macrophage polarization by silencing miR-155 - PMC

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Advanced materials (Deerfield Beach, Fla.)

Application:

IF:

32.08

Species:

Mus musculus

PMID:

37589156

Title:

Cellular MembraneEngineered Nanovesicles as ThreeStage Booster to Target Lesion Core

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

International immunopharmacology

Application:

IHC

IF:

5.6

Species:

Mus musculus

PMID:

38565043

Title:

IGF2 promotes alveolar bone regeneration in murine periodontitis via inhibiting cGAS/STING-mediated M1 macrophage polarization

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Molecular neurobiology

Application:

IF:

5.68

Species:

Mus musculus

PMID:

37525083

Title:

Intracerebral Hemorrhage-Induced Brain Injury: the Role of Lysosomal-Associated Transmembrane Protein 5

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Biomed. Mater.

Application:

IF:

4.103

Species:

Homo sapiens

PMID:

29516867

Title:

The observed difference of RAW264.7 macrophage phenotype on mineralized collagen and hydroxyapatite

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IHC

IF:

17.52

Species:

Mus musculus

PMID:

37635177

Title:

InflammationResponsive Hydrogel Spray for Synergistic Prevention of Traumatic Heterotopic Ossification via DualHomeostatic Modulation Strategy

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Research (Washington, D.C.)

Application:

IHC

IF:

Species:

Mus musculus

PMID:

39109246

Title:

Aggregation-induced emission-active photosensitizer-mediated photodynamic therapy for anti-psoriasis

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

6.1

Species:

Mus musculus

PMID:

38809142

Title:

Highly Effective NobiletinMPN in Yeast Microcapsules for Targeted Modulation of Oxidative Stress, NLRP3 Inflammasome Activation, and Immune Responses in

References for CD86 Rabbit pAb (A1199)

Product:

CD86 Rabbit pAb

Journal:

Journal of periodontology

Application:

IF:

4.3

Species:

Mus musculus

PMID:

38967396