제품 > 항체 > 단일클론항체(mAb)

CD9 Rabbit mAb (A19027)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using CD9 Rabbit mAb (A19027) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - CD9 Rabbit mAb (A19027)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using CD9 Rabbit mAb (A19027) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunoprecipitation - CD9 Rabbit mAb (A19027)

Immunoprecipitation analysis of 300 μg extracts from THP-1 cells using 3 μg CD9 Rabbit mAb (A19027). Western blot was performed from the immunoprecipitate using CD9 Rabbit mAb (A19027) at a dilution of 1:1000.

Review

Overview

Product name	CD9 Rabbit mAb
Catalog No.	A19027
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0330

Background

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 129-228 of human CD9 (P21926).
Sequence	YNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQFISDICPKKDVLETFTVKSCPDAIKEVFDNKFHIIGAVGIGIAVVMIFGMIFSMILCCAIRRNREMV
Gene ID
Swiss Prot
Synonyms	MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29; CD9
Calculated MW	25kDa
Observed MW	25kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IHC-P 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	THP-1, K-562, Mouse brain, Mouse kidney
Cellular location	Cell membrane, Membrane, Multi-pass membrane protein.
Customer validation	(Remas 、Jurkat, CK（MSC-A-）,Y-1 serum EVs) WB(Homo sapiens, Mus musculus, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

CD9 Rabbit mAb images

Western blot - CD9 Rabbit mAb (A19027)

Immunohistochemistry - CD9 Rabbit mAb (A19027)

Immunoprecipitation - CD9 Rabbit mAb (A19027)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A19027

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Reviews List

Product Protocols

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

Cancers

Application:

IF:

5.2

Species:

Homo sapiens

PMID:

36612209

Title:

Combination of Small Extracellular Vesicle-Derived Annexin A2 Protein and mRNA as a Potential Predictive Biomarker for Chemotherapy Responsiveness in Aggressive Triple-Negative Breast Cancer - PMC

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

Bioactive materials

Application:

IF:

18.9

Species:

Mus musculus

PMID:

37006825

Title:

Exosomes from young healthy human plasma promote functional recovery from intracerebral hemorrhage via counteracting ferroptotic injury - PMC

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

Cancer cell international

Application:

IF:

6.4

Species:

Mus musculus

PMID:

37268941

Title:

Local radiotherapy for murine breast cancer increases risk of metastasis by promoting the recruitment of M-MDSCs in lung - PMC

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

International journal of biological sciences

Application:

IF:

8.2

Species:

Homo sapiens

PMID:

37781038

Title:

MKL-1 suppresses ferroptosis by activating system Xc- and increasing glutathione synthesis - PMC

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

Cell reports. Medicine

Application:

IF:

11.7

Species:

Homo sapiens

PMID:

37633276

Title:

Plasma proteomic profiling discovers molecular features associated with upper tract urothelial carcinoma

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

Small

Application:

IF:

13.3

Species:

Mus musculus

PMID:

37608451

Title:

Engineered Extracellular Vesicles to Enhance Antigen Presentation for Boosting Light-Driven Tumor Immunotherapy

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

eLife

Application:

IF:

6.4

Species:

Mus musculus

PMID:

37104115

Title:

NSC-derived exosomes enhance therapeutic effects of NSC transplantation on cerebral ischemia in mice - PMC

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

Molecular and cellular endocrinology

Application:

IF:

3.8

Species:

Homo sapiens, Mus musculus

PMID:

37028587

Title:

Melatonin enhances anti-tumor immunity by targeting macrophages PD-L1 via exosomes derived from gastric cancer cells

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

Journal of diabetes

Application:

IF:

4.5

Species:

Homo sapiens, Mus musculus

PMID:

37646268

Title:

IFN enhances the therapeutic efficacy of MSCsderived exosome via miR1263p in diabetic wound healing by targeting SPRED1

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

Molecular therapy : the journal of the American Society of Gene Therapy

Application:

IF:

12.1

Species:

Mus musculus

PMID:

38098229

Title:

Irradiated engineered tumor cell-derived microparticles remodel the tumor immune microenvironment and enhance antitumor immunity

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

ACS nano

Application:

IF:

15.8

Species:

Mus musculus

PMID:

38278522

Title:

Synergistic Viro-chemoimmunotherapy in Breast Cancer Enabled by Bioengineered Immunostimulatory Exosomes and Dual-Targeted Coxsackievirus B3 - PMC

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

Journal of extracellular vesicles

Application:

IF:

Species:

Homo sapiens

PMID:

38490958

Title:

Proteomic analysis of ascitic extracellular vesicles describes tumour microenvironment and predicts patient survival in ovarian cancer

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

Adv Mater

Application:

IF:

29.4

Species:

Mus musculus

PMID:

34665481

Title:

Engineering Extracellular Vesicles Enriched with Palmitoylated ACE2 as COVID19 Therapy

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

Nature communications

Application:

IF:

16.6

Species:

Homo sapiens

PMID:

35915084

Title:

Breast cancer cell-derived extracellular vesicles promote CD8+T cell exhaustion via TGF- type II receptor signaling - PMC

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

15.1

Species:

Homo sapiens

PMID:

37712174

Title:

AdiposeDerived Mesenchymal Stem CellDerived Exosomes Biopotentiated Extracellular Matrix Hydrogels Accelerate Diabetic Wound Healing and Skin

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

Journal of controlled release : official journal of the Controlled Release Society

Application:

IF:

10.5

Species:

Mus musculus

PMID:

38643936

Title:

Hybrid Adipocyte-Derived Exosome Nano Platform for Potent Chemo-Phototherapy in Targeted Hepatocellular Carcinoma

References for CD9 Rabbit mAb (A19027)

Product:

CD9 Rabbit mAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Mus musculus

PMID:

39438865

Title:

Exosomal miR-1a-3p derived from glucocorticoid-stimulated M1 macrophages promotes the adipogenic differentiation of BMSCs in glucocorticoid-associated osteonecrosis of the femoral head by targeting Cebpz - PMC