제품 > 항체 > 다중클론항체(pAb)

CD9 Rabbit pAb (A1703)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using CD9 Rabbit pAb (A1703) at 1:7000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - CD9 Rabbit pAb (A1703)

Immunohistochemistry analysis of paraffin-embedded human smooth muscle using CD9 Rabbit pAb (A1703) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product name	CD9 Rabbit pAb
Catalog No.	A1703
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 112-195 of human CD9 (NP_001760.1).
Sequence	SHKDEVIKEVQEFYKDTYNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQFISDICPKKDVLETFTVKSCPDAIKEVFDNKFHI
Gene ID
Swiss Prot
Synonyms	MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29; CD9
Calculated MW	25kDa
Observed MW	22kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:8000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HeLa, Mouse placenta, Mouse lung, Rat uterus
Cellular location	Cell membrane, Membrane, Multi-pass membrane protein
Customer validation	WB(Homo sapiens, Mus musculus, Gallus gallus, Rattus norvegicus, Chlorocebus aethiops, Capra hircus, Sus scrofa, Other) IF(Rattus norvegicus)

Documents

Certificate of Compliance

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Research Area

CD9 Rabbit pAb images

Western blot - CD9 Rabbit pAb (A1703)

Immunohistochemistry - CD9 Rabbit pAb (A1703)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Journal of cellular physiology

Application:

IF:

5.6

Species:

Homo sapiens

PMID:

31124125

Title:

Exosomes from human adipose-derived stem cells promote sciatic nerve regeneration via optimizing Schwann cell function.

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Aging

Application:

IF:

3.9

Species:

Homo sapiens

PMID:

32096479

Title:

Adipose-derived mesenchymal stem cells-derived exosome-mediated microRNA-342-5p protects endothelial cells against atherosclerosis

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Journal of Proteome Research

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

32248692

Title:

Characterization of urinary exosomes purified with size exclusion chromatography and ultracentrifugation

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Oxid Med Cell Longev

Application:

IF:

Species:

Mus musculus

PMID:

33859780

Title:

Zoledronic Acid Enhanced the Antitumor Effect of Cisplatin on Orthotopic Osteosarcoma by ROS-PI3K/AKT Signaling and Attenuated Osteolysis

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Int Immunopharmacol

Application:

IF:

4.8

Species:

Mus musculus

PMID:

34435585

Title:

Exosomes derived from induced pluripotent stem cells suppresses M2-type macrophages during pulmonary fibrosis via miR-302a-3p/TET1 axis

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Biomaterials

Application:

IF:

12.8

Species:

Homo sapiens

PMID:

34808560

Title:

ECM stiffness-tuned exosomes drive breast cancer motility through thrombospondin-1

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Reprod Domest Anim

Application:

IF:

1.6

Species:

Gallus gallus

PMID:

34717018

Title:

Characterization and proteomics of chicken seminal plasma extracellular vesicles

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Arch Biochem Biophys

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

34774484

Title:

N-glycosylation status of Trop2 impacts its surface density, interaction with claudin-7 and exosomal release

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Journal of cellular and molecular medicine

Application:

IF:

5.3

Species:

Mus musculus

PMID:

34953015

Title:

Gingival mesenchymal stem cell‐derived exosomes are immunosuppressive in preventing collagen‐induced arthritis

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Rattus norvegicus

PMID:

36516531

Title:

Huc-MSCs-derived exosomes attenuate neuropathic pain by inhibiting activation of the TLR2/MyD88/NF-κB signaling pathway in the spinal microglia by …

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Reproductive sciences (Thousand Oaks, Calif.)

Application:

IF:

2.6

Species:

Mus musculus

PMID:

36525236

Title:

Combined Application of Exosomes and FPR2 Agonist LXA4 in Controlling Fetal Membrane Inflammation and Promoting Fetal Membrane Tissue Repair

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Cells

Application:

IF:

7.7

Species:

Mus musculus

PMID:

36552720

Title:

Transcriptome Analysis Unveils That Exosomes Derived from M1-Polarized Microglia Induce Ferroptosis of Neuronal Cells - PMC

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

7.7

Species:

Homo sapiens

PMID:

36830031

Title:

Extracellular Vesicles, as Drug-Delivery Vehicles, Improve the Biological Activities of Astaxanthin - PMC

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Open medicine (Warsaw, Poland)

Application:

IF:

Species:

Homo sapiens

PMID:

36941991

Title:

Exosomal miR-93-5p regulated the progression of osteoarthritis by targeting ADAMTS9 - PMC

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

ACS nano

Application:

IF:

15.8

Species:

Mus musculus

PMID:

37022097

Title:

Extracellular Vesicle ITGAM and ITGB2 Mediate Severe Acute Pancreatitis-Related Acute Lung Injury - PMC

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Advanced healthcare materials

Application:

IF:

Species:

Homo sapiens

PMID:

37129521

Title:

Development of a Novel DNA Aptamer Targeting Colorectal Cancer CellDerived Small Extracellular Vesicles as a Potential Diagnostic and Therapeutic Agent

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

37506505

Title:

HSF1 enhances the attenuation of exosomes from mesenchymal stem cells to hemorrhagic shock induced lung injury by altering the protein profile of

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

MedComm

Application:

IF:

9.9

Species:

Homo sapiens

PMID:

38053815

Title:

Integrated transcriptomics, proteomics, and functional analysis to characterize the tissue-specific small extracellular vesicle network of breast cancer - PMC

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Disease markers

Application:

IF:

Species:

Rattus norvegicus

PMID:

36148159

Title:

Exosomes Derived from Adipose Mesenchymal Stem Cells Carrying miRNA-22-3p Promote Schwann Cells Proliferation and Migration through Downregulation of PTEN - PMC

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

37983660

Title:

White adipose tissuederived small extracellular vesicles: A new potential therapeutic reagent for accelerating diabetic wound healing

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Clinical and translational medicine

Application:

IF:

10.6

Species:

Mus musculus

PMID:

38385857

Title:

Tumour-associated macrophage-derived DOCK7-enriched extracellular vesicles drive tumour metastasis in colorectal cancer via the RAC1/ABCA1 axis - PMC

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

MedComm

Application:

IF:

9.9

Species:

Homo sapiens

PMID:

38585233

Title:

miR-1246 promotes osteosarcoma cell migration via NamiRNA-enhancer network dependent on Argonaute 2 - PMC

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Exp Cell Res

Application:

IF:

3.3

Species:

Rattus norvegicus

PMID:

34863709

Title:

Extracellular vesicles derived from astrocytes facilitated neurite elongation by activating the Hippo pathway

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Stem cell research & therapy

Application:

WB, IF

IF:

8.1

Species:

Rattus norvegicus

PMID:

35841008

Title:

BMSC-derived exosomes promote tendon-bone healing after anterior cruciate ligament reconstruction by regulating M1/M2 macrophage polarization in rats - PMC

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Journal of virology

Application:

IF:

Species:

Chlorocebus aethiops,Gallus gallus,Homo sapiens

PMID:

38299843

Title:

Phospho-eIF4E stimulation regulates coronavirus entry by selective expression of cell membrane-residential factors

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Journal of ovarian research

Application:

IF:

5.5

Species:

Capra hircus

PMID:

38956672

Title:

Metformin modifies plasma microbial-derived extracellular vesicles in polycystic ovary syndrome with insulin resistance - PMC

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Veterinary microbiology

Application:

IF:

2.4

Species:

Sus scrofa

PMID:

38518631

Title:

Exosomes Secreted by CSFV-Infected Cells Evade Neutralizing Antibody to Activate Innate Immune Responses and Establish Productive Infection in Recipient Cells

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Vox sanguinis

Application:

IF:

1.8

Species:

Homo sapiens

PMID:

38839077

Title:

Extracellular vesicles derived from stored red blood cell suspensions enhance invasion and migration of lung cancer cells by miR1246 and miR1503p

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Poultry science

Application:

IF:

4.4

Species:

Sus scrofa

PMID:

39003794

Title:

Identification of potential candidate miRNAs related to semen quality in seminal plasma extracellular vesicles and sperms of male duck (Anas Platyrhynchos)

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Poultry science

Application:

IF:

4.4

Species:

Other

PMID:

38943808

Title:

Follicular fluid-derived exosomal HMOX1 promotes granulosa cell ferroptosis involved in follicular atresia in geese (Anser cygnoides)

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Mol Ther Nucleic Acids

Application:

IF:

6.5

Species:

Mus musculus

PMID:

34094711

Title:

Cancer-derived exosome miRNAs induce skeletal muscle wasting by Bcl-2-mediated apoptosis in colon cancer cachexia

References for CD9 Rabbit pAb (A1703)

Product:

CD9 Rabbit pAb

Journal:

Respiratory research

Application:

IF:

5.8

Species:

Other

PMID:

39342213

Title:

Differential proteins from EVs identification based on tandem mass tags analysis and effect of Treg-derived EVs on T-lymphocytes in COPD patients - PMC