제품 > 항체 > 다중클론항체(pAb)

CDK1 Rabbit pAb (A0220)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using CDK1 Rabbit pAb (A0220) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunohistochemistry - CDK1 Rabbit pAb (A0220)

Immunohistochemistry analysis of paraffin-embedded Rat brain using CDK1 Rabbit pAb (A0220) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using CDK1 Rabbit pAb (A0220) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain using CDK1 Rabbit pAb (A0220) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human esophagus using CDK1 Rabbit pAb (A0220) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using CDK1 Rabbit pAb (A0220) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - CDK1 Rabbit pAb (A0220)

Immunofluorescence analysis of 293T cells using CDK1 Rabbit pAb (A0220) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of Neuro-2a cells using CDK1 Rabbit pAb (A0220) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using CDK1 Rabbit pAb (A0220) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - CDK1 Rabbit pAb (A0220)

Immunoprecipitation analysis of 600 μg extracts of C6 cells using 3 μg CDK1 antibody (A0220). Western blot was performed from the immunoprecipitate using CDK1 antibody (A0220) at a dilution of 1:1000.

Review

Overview

Product name	CDK1 Rabbit pAb
Catalog No.	A0220
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 198-297 of human CDK1 (NP_001777.1).
Sequence	ATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM
Gene ID
Swiss Prot
Synonyms	CDC2; CDC28A; P34CDC2; CDK1
Calculated MW	34kDa
Observed MW	34kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:100 IF/ICC 1:50 - 1:200 IP 2μg antibody for 200μgextracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	Jurkat, C6, Mouse spleen
Cellular location	Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle.
Customer validation	(Human gastric cancer cells, Human tumor cell, HeLa, HCT116) WB(Homo sapiens, Rattus norvegicus, Mus musculus, Sus scrofa, Mus musculus, ) IF(Homo sapiens) IP(Homo sapiens, Rattus norvegicus) IHC(Homo sapiens, Mus musculus,) IHC(Rattus norvegicus) WB(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

CDK1 Rabbit pAb images

Western blot - CDK1 Rabbit pAb (A0220)

Immunohistochemistry - CDK1 Rabbit pAb (A0220)

Immunofluorescence - CDK1 Rabbit pAb (A0220)

Immunoprecipitation - CDK1 Rabbit pAb (A0220)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A0220

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Reviews List

Product Protocols

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Cellular physiology and biochemistry

Application:

IF:

4.644

Species:

Homo sapiens

PMID:

28214890

Title:

A New Perspective for Osteosarcoma Therapy: Proteasome Inhibition by MLN9708/2238 Successfully Induces Apoptosis and Cell Cycle Arrest and Attenuates the Invasion Ability of Osteosarcoma Cells in Vitro

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Nutrition and cancer

Application:

IF:

2.9

Species:

Homo sapiens

PMID:

30358420

Title:

Modulation of VDR and Cell Cycle-Related Proteins by Vitamin D in Normal Pancreatic Cells and Poorly Differentiated Metastatic Pancreatic Cancer Cells.

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

European journal of pharmacology

Application:

IF:

4.432

Species:

Rattus norvegicus

PMID:

31103630

Title:

Simvastatin inhibits the proliferation of HL-60 clone 15- derived eosinophils by inducing the arrest of the cell cycle in the G1/S phase.

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

OncoTargets and therapy

Application:

IF:

4.147

Species:

Homo sapiens

PMID:

31749621

Title:

Se-Enriched Cordyceps militaris Inhibits Cell Proliferation, Induces Cell Apoptosis, And Causes G2/M Phase Arrest In Human Non-Small Cell Lung Cancer Cells.

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Onco Targets Ther

Application:

IF:

4.147

Species:

Homo sapiens

PMID:

33116610

Title:

Analysis of Differential Expression Proteins of Paclitaxel-Treated Lung Adenocarcinoma Cell A549 Using Tandem Mass Tag-Based Quantitative Proteomics

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

BMC Med Genomics

Application:

IF:

3.06

Species:

Homo sapiens

PMID:

34587952

Title:

Identification and validation of pivotal genes related to age-related meniscus degeneration based on gene expression profiling analysis and in vivo and in vitro models detection

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Biochem Pharmacol

Application:

IF:

5.858

Species:

Homo sapiens

PMID:

34111425

Title:

Inhibition of Pim-2 kinase by LT-171-861 promotes DNA damage and exhibits enhanced lethal effects with PARP Inhibitor in multiple myeloma

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Microbiol Spectr

Application:

IF:

7.171

Species:

Mus musculus

PMID:

34756071

Title:

Japanese Encephalitis Virus NS1′ Protein Interacts with Host CDK1 Protein to Regulate Antiviral Response

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Metallomics : integrated biometal science

Application:

IF:

4.63

Species:

Rattus norvegicus, Sus scrofa

PMID:

33237045

Title:

Selenium supplementation protects against oxidative stress-induced cardiomyocyte cell cycle arrest through activation of PI3K/AKT

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Molecular medicine reports

Application:

IF:

3.42

Species:

Homo sapiens

PMID:

33398375

Title:

miR-613 inhibits the proliferation of human ovarian granulosa cells by arresting cell cycle progression via the targeting of IGF-1

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Homo sapiens

PMID:

35682967

Title:

Carboplatin-Induced Thrombocytopenia through JAK2 Downregulation, S-Phase Cell Cycle Arrest and Apoptosis in Megakaryocytes - PMC

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Anticancer research

Application:

IF:

2.43

Species:

Homo sapiens

PMID:

36854527

Title:

Hinokitiol Inhibits the Viability of Oral Squamous Carcinoma Cells by Inducing Apoptosis and Autophagy

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Cell reports

Application:

IF:

Species:

Homo sapiens

PMID:

36995937

Title:

CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Molecular medicine (Cambridge, Mass.)

Application:

IF:

6.37

Species:

Homo sapiens

PMID:

36977984

Title:

The promotion action of AURKA on post-ischemic angiogenesis in diabetes-related limb ischemia - PMC

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Oncology letters

Application:

IF:

3.11

Species:

Homo sapiens

PMID:

37153047

Title:

AURKA, TOP2A and MELK are the key genes identified by WGCNA for the pathogenesis of lung adenocarcinoma - PMC

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Application:

IF:

3.1

Species:

Rattus norvegicus

PMID:

37442643

Title:

Leukocytepoor plateletrich plasma and leukocyterich plateletrich plasma promote myoblast proliferation through the upregulation of cyclin A, cdk1, and cdk2

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Mycotoxin research

Application:

IF:

4.08

Species:

Homo sapiens

PMID:

37400696

Title:

Fusaric acid inhibits proliferation and induces apoptosis through triggering endoplasmic reticulum stress in MCF-7 human breast cancer cells

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Experimental cell research

Application:

IF:

4.14

Species:

Homo sapiens

PMID:

35405117

Title:

Deubiquitinating enzyme PSMD14 facilitates gastric carcinogenesis through stabilizing PTBP1

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Oncology reports

Application:

IF:

4.13

Species:

Homo sapiens, Mus musculus

PMID:

35866591

Title:

Overexpressed lncRNA FTX promotes the cell viability, proliferation, migration and invasion of renal cell carcinoma via FTX/miR-4429/UBE2C axis - PMC

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Nature chemical biology

Application:

WB, IP

IF:

16.17

Species:

Homo sapiens

PMID:

36635566

Title:

SIRT1 deacetylates WEE1 and sensitizes cancer cells to WEE1 inhibition

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Journal of genetics and genomics = Yi chuan xue bao

Application:

IF:

5.72

Species:

Mus musculus

PMID:

37481122

Title:

LSM14B coordinates protein component expression in the P-body and controls oocyte maturation

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Biomarker research

Application:

IF:

8.63

Species:

Homo sapiens

PMID:

38273343

Title:

Simultaneous inhibition of FAK and ROS1 synergistically repressed triple-negative breast cancer by upregulating p53 signalling - PMC

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Cardiovascular toxicology

Application:

IF:

2.75

Species:

Mus musculus

PMID:

38310188

Title:

Blockade of ZFX Alleviates Hypoxia-Induced Pulmonary Vascular Remodeling by Regulating the YAP Signaling

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Platelets

Application:

IF:

4.23

Species:

Homo sapiens

PMID:

27780401

Title:

Platelet rich plasma releasate promotes proliferation of skeletal muscle cells in association with upregulation of PCNA, cyclins and cyclin dependent kinases

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Toxicological Sciences

Application:

WB, IP

IF:

4.849

Species:

Rattus norvegicus

PMID:

26577051

Title:

Prevention of Simvastatin-Induced Inhibition of Tendon Cell Proliferation and Cell Cycle Progression by Geranylgeranyl Pyrophosphate

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

PLoS pathogens

Application:

IF:

7.46

Species:

Mus musculus

PMID:

36656913

Title:

Suppression of KSHV lytic replication and primary effusion lymphoma by selective RNF5 inhibition - PMC

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

European journal of medicinal chemistry

Application:

WB, IHC

IF:

7.08

Species:

Homo sapiens, Mus musculus,

PMID:

37837671

Title:

Design, synthesis, and biological evaluation of novel 4, 4-bipyridine derivatives acting as CDK9-Cyclin T1 protein-protein interaction inhibitors against triple

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Cancer biotherapy & radiopharmaceuticals

Application:

IF:

3.4

Species:

Homo sapiens

PMID:

38949986

Title:

Stratification of Lung Adenocarcinoma Patients Based on In Silico and Immunohistochemistry Analyses of Oxidative Stress-Related Genes

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Heliyon

Application:

IF:

Species:

Homo sapiens,Mus musculus

PMID:

39027542

Title:

Astragaloside Ⅳ mediates the effect and mechanism of KPNB1 on biological behavior and tumor growth in prostate cancer - PMC

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.6

Species:

Mus musculus

PMID:

39125009

Title:

Fucoxanthin Inhibits the Proliferation and Metastasis of Human Pharyngeal Squamous Cell Carcinoma by Regulating the PI3K/Akt/mTOR Signaling Pathway - PMC

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

iScience

Application:

IF:

5.8

Species:

Mus musculus

PMID:

39184442

Title:

Astroglia proliferate upon the biogenesis of tunneling nanotubes via -synuclein dependent transient nuclear translocation of focal adhesion kinase - PMC

References for CDK1 Rabbit pAb (A0220)

Product:

CDK1 Rabbit pAb

Journal:

Acta Pharmacologica Sinica

Application:

IF:

8.2

Species:

Mus musculus

PMID:

31316178