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CDKN1A/p21CIP1 Rabbit pAb (A1483)

Reviews (3)Publications (65) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Western blot analysis of lysates from MCF7 cells, using CDKN1A/p21CIP1 Rabbit pAb (A1483) at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Western blot analysis of various lysates, using CDKN1A/p21CIP1 Rabbit pAb (A1483) at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using CDKN1A/p21CIP1 Rabbit pAb (A1483) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of C6 cells using CDKN1A/p21CIP1 Rabbit pAb (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of HeLa cells using CDKN1A/p21CIP1 Rabbit pAb (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of NIH/3T3 cells using CDKN1A/p21CIP1 Rabbit pAb (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for CDKN1A/p21CIP1 Rabbit pAb(A1483)
Review
ABclonal: review for CDKN1A/p21CIP1 Rabbit pAb(A1483)
Review
ABclonal: review for CDKN1A/p21CIP1 Rabbit pAb(A1483)

Overview

Product nameCDKN1A/p21CIP1 Rabbit pAb
Catalog No.A1483
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-164 of human CDKN1A/p21CIP1 (NP_000380.1).
SequenceMSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP
Gene ID
Swiss Prot
SynonymsP21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1; CDKN1A/p21CIP1
Calculated MW18kDa
Observed MW18kDa(Mouse)/21kDa(Human/Rat)
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, Mouse large intestine, Mouse testis, Rat lung
Cellular locationCytoplasm, Nucleus.
Customer validation

(rat cells, P19, P21)

WB(Homo sapiens, Mus musculus, Sanghuangporus vaninii, Rattus norvegicus, Mus musculus, Ovis aries)

IF(Rattus norvegicus, Homo sapiens, Gallus gallus)

IHC(Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus)

ChIP(Rattus norvegicus)

IHC(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)}

Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Western blot analysis of lysates from MCF7 cells, using CDKN1A/p21CIP1 Rabbit pAb (A1483) at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)}

Western blot - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Western blot analysis of various lysates, using CDKN1A/p21CIP1 Rabbit pAb (A1483) at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - CDKN1A/p21CIP1 Rabbit pAb (A1483)}

Immunohistochemistry - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using CDKN1A/p21CIP1 Rabbit pAb (A1483) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)}

Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of C6 cells using CDKN1A/p21CIP1 Rabbit pAb (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)}

Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of HeLa cells using CDKN1A/p21CIP1 Rabbit pAb (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)}

Immunofluorescence - CDKN1A/p21CIP1 Rabbit pAb (A1483)

Immunofluorescence analysis of NIH/3T3 cells using CDKN1A/p21CIP1 Rabbit pAb (A1483) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (3)

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A19094

WBIHC-PELISA

Reactivity:Human, Mouse, Rat

[KO Validated] CDKN1A/p21 Rabbit mAb

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A2691

WBIHC-PIPELISA

Reactivity:Human, Mouse

CDKN1A/p21CIP1 Rabbit pAb

CDKN1A/p21CIP1 Rabbit mAb
A21897

WBIHC-PELISA

Reactivity:Human

CDKN1A/p21CIP1 Rabbit mAb

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)