제품 > 항체 > 다중클론항체(pAb)

CEBPA Rabbit pAb (A0904)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using CEBPA Rabbit pAb (A0904) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - CEBPA Rabbit pAb (A0904)

Immunohistochemistry analysis of paraffin-embedded Rat liver using CEBPA Rabbit pAb (A0904) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using CEBPA Rabbit pAb (A0904) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver using CEBPA Rabbit pAb (A0904) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	CEBPA Rabbit pAb
Catalog No.	A0904
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 111-180 of human CEBPA (NP_004355.2).
Sequence	APAGPGGAVMPGGAHGPPPGYGCAAAGYLDGRLEPLYERVGAPALRPLVIKQEPREEDEAKQLALAGLFP
Gene ID
Swiss Prot
Synonyms	CEBP; C/EBP-alpha; CEBPA
Calculated MW	38kDa
Observed MW	30kDa/42kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	LO2, Mouse kidney, Rat lung, Rat kidney
Cellular location	Nucleus, Nucleus, nucleolus.
Customer validation	WB(Homo sapiens, Sus scrofa, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus) RT-qPCR(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

CEBPA Rabbit pAb images

Western blot - CEBPA Rabbit pAb (A0904)

Immunohistochemistry - CEBPA Rabbit pAb (A0904)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for CEBPA Rabbit pAb (A0904)

Product:

CEBPA Rabbit pAb

Journal:

Experimental Cell Research

Application:

IF:

3.905

Species:

Homo sapiens

PMID:

31837293

Title:

Knock-down of circular RNA H19 induces human adipose-derived stem cells adipogenic differentiation via a mechanism involving the polypyrimidine tract-binding protein 1

References for CEBPA Rabbit pAb (A0904)

Product:

CEBPA Rabbit pAb

Journal:

Genomics

Application:

IF:

5.736

Species:

Sus scrofa

PMID:

32027955

Title:

The transcriptomes from two adipocyte progenitor cell types provide insight into the differential functions of MSTN

References for CEBPA Rabbit pAb (A0904)

Product:

CEBPA Rabbit pAb

Journal:

Anal Chem

Application:

IF:

6.986

Species:

Homo sapiens

PMID:

33596054

Title:

CD36 as a Molecular Target of Functional DNA Aptamer NAFLD01 Selected against NAFLD Cells

References for CEBPA Rabbit pAb (A0904)

Product:

CEBPA Rabbit pAb

Journal:

Cellular and molecular gastroenterology and hepatology

Application:

IF:

8.79

Species:

Mus musculus

PMID:

35569816

Title:

Human Cytomegalovirus-IE2 Affects Embryonic Liver Development and Survival in Transgenic Mouse - PMC

References for CEBPA Rabbit pAb (A0904)

Product:

CEBPA Rabbit pAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Mus musculus

PMID:

32432606

Title:

Medium-, long- and medium-chain-type structured lipids ameliorate high-fat diet-induced atherosclerosis by regulating inflammation, adipogenesis, and gut microbiota in ApoE -/- mice

References for CEBPA Rabbit pAb (A0904)

Product:

CEBPA Rabbit pAb

Journal:

Biotechnology & genetic engineering reviews

Application:

IF:

4.2

Species:

Mus musculus

PMID:

37010292

Title:

Analysis of the role of irisin receptor signaling in regulating osteogenic/adipogenic differentiation of bone marrow mesenchymal stem cells

References for CEBPA Rabbit pAb (A0904)

Product:

CEBPA Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Oryctolagus cuniculus

PMID:

37762324

Title:

miR-383-5p Regulates Preadipocyte Proliferation and Differentiation by Targeting RAD51AP1 - PMC

References for CEBPA Rabbit pAb (A0904)

Product:

CEBPA Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Oryctolagus cuniculus

PMID:

37958699

Title:

lncRNA MSTRG4710 Promotes the Proliferation and Differentiation of Preadipocytes through miR-29b-3p/IGF1 Axis - PMC

References for CEBPA Rabbit pAb (A0904)

Product:

CEBPA Rabbit pAb

Journal:

iScience

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

36594018

Title:

Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice - PMC

References for CEBPA Rabbit pAb (A0904)

Product:

CEBPA Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

7.5

Species:

Mus musculus

PMID:

38460364

Title:

Dendrobium officinale regulate lipid metabolism in diabetic mouse liver via PPAR-RXR signaling pathway: Evidence from an integrated multi-omics analysis

References for CEBPA Rabbit pAb (A0904)

Product:

CEBPA Rabbit pAb

Journal:

Phytotherapy research : PTR

Application:

WB, RT-qPCR

IF:

6.38

Species:

Rattus norvegicus

PMID:

36799485

Title:

Peiminine regulates bonefat balance by canonical Wnt/catenin pathway in an ovariectomized rat model

References for CEBPA Rabbit pAb (A0904)

Product:

CEBPA Rabbit pAb

Journal:

Orthopaedic journal of sports medicine

Application:

IF:

2.6

Species:

Homo sapiens

PMID:

38840789

Title:

Influence of Frequent Corticosteroid Local Injections on the Expression of Genes and Proteins Related to Fatty Infiltration, Muscle Atrophy, Inflammation, and Fibrosis in Patients With Chronic Rotator Cuff Tears: A Pilot Study - PMC

References for CEBPA Rabbit pAb (A0904)

Product:

CEBPA Rabbit pAb

Journal:

Adipocyte

Application:

IF:

3.3

Species:

Oryctolagus cuniculus

PMID:

38858810