제품 > 항체 > 다중클론항체(pAb)

CHRM1 Rabbit pAb (A16819)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CHRM1 Rabbit pAb (A16819)

Western blot analysis of various lysates using CHRM1 Rabbit pAb (A16819) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - CHRM1 Rabbit pAb (A16819)

Immunofluorescence analysis of OVCAR-3 cells using CHRM1 Rabbit pAb (A16819) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CHRM1 Rabbit pAb (A16819)

Immunofluorescence analysis of C6 cells using CHRM1 Rabbit pAb (A16819) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CHRM1 Rabbit pAb (A16819)

Immunofluorescence analysis of NIH-3T3 cells using CHRM1 Rabbit pAb (A16819) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CHRM1 Rabbit pAb (A16819)

Immunofluorescence analysis of OVCAR-3 cells using CHRM1 Rabbit pAb (A16819) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCHRM1 Rabbit pAb
Catalog No.A16819
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human CHRM1 (NP_000729.2).
SequenceMNTSAPPAVSPNITVLAPGKGPWQVAFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDL
Gene ID
Swiss Prot
SynonymsM1; HM1; M1R; CHRM1
Calculated MW51kDa
Observed MW51kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular locationCell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse.
Customer validation

IHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - CHRM1 Rabbit pAb (A16819)}

Western blot - CHRM1 Rabbit pAb (A16819)

Western blot analysis of various lysates using CHRM1 Rabbit pAb (A16819) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - CHRM1 Rabbit pAb (A16819)}

Immunofluorescence - CHRM1 Rabbit pAb (A16819)

Immunofluorescence analysis of OVCAR-3 cells using CHRM1 Rabbit pAb (A16819) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CHRM1 Rabbit pAb (A16819)}

Immunofluorescence - CHRM1 Rabbit pAb (A16819)

Immunofluorescence analysis of C6 cells using CHRM1 Rabbit pAb (A16819) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CHRM1 Rabbit pAb (A16819)}

Immunofluorescence - CHRM1 Rabbit pAb (A16819)

Immunofluorescence analysis of NIH-3T3 cells using CHRM1 Rabbit pAb (A16819) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CHRM1 Rabbit pAb (A16819)}

Immunofluorescence - CHRM1 Rabbit pAb (A16819)

Immunofluorescence analysis of OVCAR-3 cells using CHRM1 Rabbit pAb (A16819) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A16819? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (25)