Review (1)Publications (30) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse
Western blot analysis of lysates from Mouse skeletal muscle, using COL2A1 Rabbit pAb (A1560) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human cartilage tissue using COL2A1 Rabbit pAb (A1560) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of HepG2 cells using COL2A1 Rabbit pAb (A1560) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
| Product name | COL2A1 Rabbit pAb |
|---|---|
| Catalog No. | A1560 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1220-1282 of human COL2A1 (NP_001835.3). |
|---|---|
| Sequence | MSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPART |
| Gene ID | |
| Swiss Prot | |
| Synonyms | AOM; ANFH; SEDC; STL1; COL11A3; COL2A1 |
| Calculated MW | 142kDa |
| Observed MW | 141kDa |
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | Mouse skeletal muscle |
| Cellular location | Secreted, extracellular matrix, extracellular space. |
| Customer validation | (Mouse intervertebral disc) WB(Rattus norvegicus, Gallus gallus, Homo sapiens, Mus musculus) IHC(Rattus norvegicus, Homo sapiens, Mus musculus) IF(Mus musculus , Canis familiaris, Homo sapiens, Rattus norvegicus) IF(Homo sapiens, Gallus gallus, Rattus norvegicus) WB(Mus musculus) IHC(Mus musculus, Rattus norvegicus, Gallus gallus) IHC(Mus musculus, Canis familiaris) |
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