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COX2/PTGS2 Rabbit pAb (A1253)

Reviews (3)Publications (120) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - COX2/PTGS2 Rabbit pAb (A1253)

Western blot analysis of various lysates using COX2/PTGS2 Rabbit pAb (A1253) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit pAb (A1253)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using COX2/PTGS2 Rabbit pAb (A1253) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit pAb (A1253)

Immunohistochemistry analysis of paraffin-embedded Human colon using COX2/PTGS2 Rabbit pAb (A1253) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Review
ABclonal: review for COX2/PTGS2 Rabbit pAb(A1253)
Review
ABclonal: review for COX2/PTGS2 Rabbit pAb(A1253)
Review
ABclonal: review for COX2/PTGS2 Rabbit pAb(A1253)

Overview

Product nameCOX2/PTGS2 Rabbit pAb
Catalog No.A1253
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 375-604 of COX2/PTGS2 (NP_000954.1).
SequencePLLPDTFQIHDQKYNYQQFIYNNSILLEHGITQFVESFTRQIAGRVAGGRNVPPAVQKVSQASIDQSRQMKYQSFNEYRKRFMLKPYESFEELTGEKEMSAELEALYGDIDAVELYPALLVEKPRPDAIFGETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNNVKGCPFTSFSVPDPELIKTVTINASSSRSGLDDINPTVLLKERSTEL
Gene ID
Swiss Prot
SynonymsCOX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS; COX2/PTGS2
Calculated MW69kDa
Observed MW72kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse uterus, Mouse lung
Cellular locationEndoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein.
Customer validation

(Mouse embryonal fibroblast cell, RAW264.7,Mouse kidney)

WB(Mus musculus, Rattus norvegicus, Schisandra chinensis, Trifolium pratense, Gallus gallus, Homo sapiens, Sus scrofa, Carassius gibelio, Mus musculus, Anser cygnoides, Danio rerio, Bos taurus)

IHC(Rattus norvegicus, Mus musculus, Homo sapiens, Mus musculus)

IHC(Rattus norvegicus, Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

IF(Mus musculus, Gallus gallus)

WB(Gallus gallus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - COX2/PTGS2 Rabbit pAb (A1253)}

Western blot - COX2/PTGS2 Rabbit pAb (A1253)

Western blot analysis of various lysates using COX2/PTGS2 Rabbit pAb (A1253) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit pAb (A1253)}

Immunohistochemistry - COX2/PTGS2 Rabbit pAb (A1253)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using COX2/PTGS2 Rabbit pAb (A1253) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit pAb (A1253)}

Immunohistochemistry - COX2/PTGS2 Rabbit pAb (A1253)

Immunohistochemistry analysis of paraffin-embedded Human colon using COX2/PTGS2 Rabbit pAb (A1253) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (3)

COX2/PTGS2 Rabbit mAb
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WBIHC-PELISA

Reactivity:Human, Mouse, Rat

COX2/PTGS2 Rabbit mAb

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A24438

WB

Reactivity:Human

PGHS-2 Rabbit pAb

COX2/PTGS2 Rabbit PolymAb®
A25901PM

WBIHC-PICC/IFIPELISA

Reactivity:Human, Mouse

COX2/PTGS2 Rabbit PolymAb®

재조합 단백질 (1)

Recombinant Human COX-2 Protein
RP02992

Tags:C-His

Expressed Host:Baculovirus-Insect Cells

Recombinant Human COX-2 Protein

ELISA 키트 (1)

Human Cyclooxygenase-2 (COX-2) ELISA Kit
RK02157

Human Cyclooxygenase-2 (COX-2) ELISA Kit

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)