제품 > 항체 > 단일클론항체(mAb)

COX IV Rabbit mAb (A11631)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using COX IV Rabbit mAb (A11631) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Immunohistochemistry - COX IV Rabbit mAb (A11631)

Immunohistochemistry analysis of paraffin-embedded Human colon using COX IV Rabbit mAb (A11631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human kidney using COX IV Rabbit mAb (A11631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using COX IV Rabbit mAb (A11631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using COX IV Rabbit mAb (A11631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using COX IV Rabbit mAb (A11631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	COX IV Rabbit mAb
Catalog No.	A11631
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2518

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human COX IV (P13073).
Sequence	MLATRVFSLVGKRAISTSVCVRAHESVVKSEDFSLPAYMDRRDHPLPEVAHVKHLSASQKALKEKEKASWSSLSMDEKVELYRIKFKESFAEMNRGSNEW
Gene ID
Swiss Prot
Synonyms	COX4; COXIV; COX4-1; COXIV-1; MC4DN16; COX IV-1; COX IV
Calculated MW	20kDa
Observed MW	17kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IHC-P 1:200 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HeLa, MCF7, Mouse brain, Mouse heart, Rat heart
Cellular location	Mitochondrion inner membrane.
Customer validation	WB(Rattus norvegicus, Homo sapiens, Cyprinus carpio, Mus musculus) IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

COX IV Rabbit mAb images

Western blot - COX IV Rabbit mAb (A11631)

Immunohistochemistry - COX IV Rabbit mAb (A11631)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for COX IV Rabbit mAb (A11631)

Product:

COX IV Rabbit mAb

Journal:

J Transl Med

Application:

IF:

5.531

Species:

Rattus norvegicus

PMID:

34538249

Title:

A new Mfn-2 related synthetic peptide promotes vascular smooth muscle cell apoptosis via regulating the mitochondrial apoptotic pathway by inhibiting Akt signaling

References for COX IV Rabbit mAb (A11631)

Product:

COX IV Rabbit mAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.65

Species:

Mus musculus

PMID:

35908522

Title:

Ban-xia-xie-xin-tang ameliorates hepatic steatosis by regulating Cidea and Cidec expression in HFD-fed mice

References for COX IV Rabbit mAb (A11631)

Product:

COX IV Rabbit mAb

Journal:

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Application:

IF:

5.57

Species:

Homo sapiens

PMID:

36208653

Title:

Apoptosis induction in human prostate cancer cells related to the fatty acid metabolism by wogonin-mediated regulation of the AKT-SREBP1-FASN signaling network

References for COX IV Rabbit mAb (A11631)

Product:

COX IV Rabbit mAb

Journal:

International journal of biological macromolecules

Application:

IF:

8.02

Species:

Homo sapiens

PMID:

37729985