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Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb (A9308)

Reviews (2)Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb (A9308)

Western blot analysis of various lysates using Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb (A9308) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb (A9308)

Immunohistochemistry analysis of paraffin-embedded Rat liver using Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb (A9308) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Review
ABclonal: review for Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb(A9308)
Review
ABclonal: review for Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb(A9308)

Overview

Product nameCasein Kinase 1 alpha (CSNK1A1) Rabbit mAb
Catalog No.A9308
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1860
Enables protein serine/threonine kinase activity. Involved in several processes, including negative regulation of canonical Wnt signaling pathway; peptidyl-serine phosphorylation; and positive regulation of proteasomal ubiquitin-dependent protein catabolic process. Located in centrosome; cytosol; and nuclear speck. Part of beta-catenin destruction complex. Colocalizes with keratin filament and mRNA cleavage and polyadenylation specificity factor complex. Biomarker of Alzheimer's disease and inclusion body myositis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 238-337 of human Casein Kinase 1 alpha (CSNK1A1) (P48729).
SequenceEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF
Gene ID
Swiss Prot
SynonymsCK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p; Casein Kinase 1 alpha (CSNK1A1)
Calculated MW39kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, NIH/3T3, Jurkat, Mouse lung, Rat lung, Rat brain
Cellular locationChromosome, Cytoplasm, Nucleus speckle, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, cilium basal body.
Customer validation

(HCT116)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb (A9308)}

Western blot - Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb (A9308)

Western blot analysis of various lysates using Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb (A9308) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb (A9308)}

Immunohistochemistry - Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb (A9308)

Immunohistochemistry analysis of paraffin-embedded Rat liver using Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb (A9308) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)