제품 > 항체 > 다중클론항체(pAb)

Caspase-12 Rabbit pAb (A0217)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Mouse liver, using Caspase-12 Rabbit pAb (A0217) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Caspase-12 Rabbit pAb (A0217)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Caspase-12 Rabbit pAb (A0217) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Caspase-12 Rabbit pAb (A0217) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat spleen using Caspase-12 Rabbit pAb (A0217) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	Caspase-12 Rabbit pAb
Catalog No.	A0217
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Caspase-12 (NP_001177945.2).
Sequence	FNNRNCQSLKDKPKVIIMQACRGNGAGIVWFTTDSGKASADTHGRLLQGNICNDAVTKAHVEKDFIAFKSSTPHNVSWRHETNGSVFISQIIYYFREYSWS
Gene ID
Swiss Prot
Synonyms	CASP-12; CASP12P1; Caspase-12
Calculated MW	39kDa
Observed MW	55kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Mouse liver
Cellular location	AIM2 inflammasome complex, cytoplasm, endoplasmic reticulum, IPAF inflammasome complex, NLRP3 inflammasome complex.
Customer validation	WB(Sus scrofa, Homo sapiens, Mus musculus, Rattus norvegicus, Canis lupus familiaris) IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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Research Area

Caspase-12 Rabbit pAb images

Western blot - Caspase-12 Rabbit pAb (A0217)

Immunohistochemistry - Caspase-12 Rabbit pAb (A0217)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Caspase-12 Rabbit pAb (A0217)

Product:

Caspase-12 Rabbit pAb

Journal:

Journal of Agricultural and Food Chemistry

Application:

IF:

5.279

Species:

Sus scrofa

PMID:

32208605

Title:

Sodium Butyrate Protects the Intestinal Barrier by Modulating Intestinal Host Defense Peptide Expression and Gut Microbiota after a Challenge with Deoxynivalenol in Weaned Piglets

References for Caspase-12 Rabbit pAb (A0217)

Product:

Caspase-12 Rabbit pAb

Journal:

Journal of Hazardous Materials

Application:

IF:

10.588

Species:

Homo sapiens

PMID:

32200236

Title:

Ambient particulate matter triggers dysfunction of subcellular structures and endothelial cell apoptosis through disruption of redox equilibrium and calcium homeostasis

References for Caspase-12 Rabbit pAb (A0217)

Product:

Caspase-12 Rabbit pAb

Journal:

Toxins

Application:

IF:

4.546

Species:

Sus scrofa

PMID:

30558299

Title:

Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells.

References for Caspase-12 Rabbit pAb (A0217)

Product:

Caspase-12 Rabbit pAb

Journal:

Molecular Medicine Reports

Application:

IHC

IF:

2.952

Species:

Rattus norvegicus

PMID:

31545437

Title:

Effects of thymosin β4 on neuronal apoptosis in a rat model of cerebral ischemia?reperfusion injury.

References for Caspase-12 Rabbit pAb (A0217)

Product:

Caspase-12 Rabbit pAb

Journal:

Cardiovasc Diagn Ther

Application:

IF:

2.845

Species:

Mus musculus

PMID:

32695629

Title:

Up-regulation of PERK/Nrf2/HO-1 axis protects myocardial tissues of mice from damage triggered by ischemia-reperfusion through ameliorating endoplasmic reticulum stress

References for Caspase-12 Rabbit pAb (A0217)

Product:

Caspase-12 Rabbit pAb

Journal:

Science Of The Total Environment

Application:

IF:

7.963

Species:

Homo sapiens

PMID:

33243499

Title:

Atmospheric particulate matter impedes autophagic flux by impairing lysosomal milieu and integrity in human umbilical vein endothelial cells (HUVECs)

References for Caspase-12 Rabbit pAb (A0217)

Product:

Caspase-12 Rabbit pAb

Journal:

World J Diabetes

Application:

IF:

3.76

Species:

Rattus norvegicus

PMID:

33384768

Title:

Effect of liraglutide on endoplasmic reticulum stress in the renal tissue of type 2 diabetic rats

References for Caspase-12 Rabbit pAb (A0217)

Product:

Caspase-12 Rabbit pAb

Journal:

Oxid Med Cell Longev

Application:

IF:

6.543

Species:

Homo sapiens

PMID:

34790286

Title:

Circular RNA circPHKA2 Relieves OGD-Induced Human Brain Microvascular Endothelial Cell Injuries through Competitively Binding miR-574-5p to Modulate SOD2

References for Caspase-12 Rabbit pAb (A0217)

Product:

Caspase-12 Rabbit pAb

Journal:

The Science of the total environment

Application:

IF:

10.75

Species:

Homo sapiens

PMID:

32050373