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Caspase-1 Rabbit pAb (A0964)

Reviews (3)Publications (233) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Caspase-1 Rabbit pAb
ABclonal:Western blot - Caspase-1 Rabbit pAb (A0964)

Western blot analysis of various lysates, using CASP1 Rabbit pAb (A0964) at 1:1800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Western blot - Caspase-1 Rabbit pAb (A0964)

Western blot analysis of various lysates, using CASP1 Rabbit pAb (A0964) at 1:1800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - Caspase-1 Rabbit pAb (A0964)

Confocal immunofluorescence analysis of Raw264.7 cells using Caspase-1 Rabbit pAb (A0964) at dilution of 1:200. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Caspase-1 Rabbit pAb (A0964)

Immunofluorescence analysis of PC-12 cells using CASP1 Rabbit pAb (A0964) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Caspase-1 Rabbit pAb (A0964)

Immunofluorescence analysis of NIH/3T3 cells using Caspase-1 Rabbit pAb (A0964) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Caspase-1 Rabbit pAb (A0964)

Immunofluorescence analysis of U2OS cells using Caspase-1 Rabbit pAb (A0964) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Caspase-1 Rabbit pAb(A0964)
Review
ABclonal: review for Caspase-1 Rabbit pAb(A0964)
Review
ABclonal: review for Caspase-1 Rabbit pAb(A0964)

Overview

Product nameCaspase-1 Rabbit pAb
Catalog No.A0964
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 201-300 of human Caspase 1 (NP_150634.1).
SequenceDVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPDILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGVVWFKDSVGVSGNLSLPTTEEFEDDAIKKAHIEKDFIAFCSSTPDNVSWRHPTMGSVFIGRLIEHMQEYACSCDVEEIFRKVRFSFEQPDGRAQMPTTERVTLTRCFYLFPGH
Gene ID
Swiss Prot
SynonymsICE; P45; IL1BC; Caspase-1
Calculated MW45kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2, Hela, Mouse lung, Mouse spleen
Cellular locationCytoplasm.
Customer validation

(Human colon cancer cell, Human chronic myeloid leukemia cells, N9)

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Sus scrofa, Danio rerio, Gallus gallus, Cyprinus carpio, Ctenopharyngodon idella, Mus musculus, Rattus norvegicus, Other)

IF(Mus musculus, Homo sapiens, Rattus norvegicus)

IHC(Rattus norvegicus, Mus musculus, Homo sapiens, Mus musculus)

WB，IHC(Rattus norvegicus)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

IHC(Mus musculus, Rattus norvegicus)

ELISA(Mus musculus, Homo sapiens)

qPCR(Mus musculus)

FC(Homo sapiens)

IP(Homo sapiens)

WB(Homo sapiens, Mus musculus)

IP(Mus musculus)

RT-qPCR(Homo sapiens)

IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Caspase-1 Rabbit pAb (A0964)}

Western blot - Caspase-1 Rabbit pAb (A0964)

Western blot analysis of various lysates, using CASP1 Rabbit pAb (A0964) at 1:1800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
ABclonal:Western blot - Caspase-1 Rabbit pAb (A0964)}

Western blot - Caspase-1 Rabbit pAb (A0964)

Western blot analysis of various lysates, using CASP1 Rabbit pAb (A0964) at 1:1800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - Caspase-1 Rabbit pAb (A0964)}

Immunofluorescence - Caspase-1 Rabbit pAb (A0964)

Confocal immunofluorescence analysis of Raw264.7 cells using Caspase-1 Rabbit pAb (A0964) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Caspase-1 Rabbit pAb (A0964)}

Immunofluorescence - Caspase-1 Rabbit pAb (A0964)

Immunofluorescence analysis of PC-12 cells using CASP1 Rabbit pAb (A0964) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Caspase-1 Rabbit pAb (A0964)}

Immunofluorescence - Caspase-1 Rabbit pAb (A0964)

Immunofluorescence analysis of NIH/3T3 cells using Caspase-1 Rabbit pAb (A0964) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Caspase-1 Rabbit pAb (A0964)}

Immunofluorescence - Caspase-1 Rabbit pAb (A0964)

Immunofluorescence analysis of U2OS cells using Caspase-1 Rabbit pAb (A0964) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (3)

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Reactivity:Human

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ELISA 키트 (1)

Human Caspase-1 (Casp1) ELISA Kit
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Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)