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Caspase-3 Rabbit pAb (A0214)

Reviews (3)Publications (60) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Caspase-3 Rabbit pAb (A0214)

Western blot analysis of various lysates using Caspase-3 Rabbit pAb (A0214) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Review
ABclonal: review for Caspase-3 Rabbit pAb(A0214)
Review
ABclonal: review for Caspase-3 Rabbit pAb(A0214)
Review
ABclonal: review for Caspase-3 Rabbit pAb(A0214)

Overview

Product nameCaspase-3 Rabbit pAb
Catalog No.A0214
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase-3 (NP_004337.2).
SequenceFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFII
Gene ID
Swiss Prot
SynonymsCPP32; SCA-1; CPP32B; Caspase-3
Calculated MW32kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:300
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, 293T
Cellular locationCytoplasm.
Customer validation

(Eoma cell, U2OS,Saos-2)

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Bos taurus, Apostichopus japonicus, Dianella ensifolia, Eriocheir sinensis, Anser cygnoides, Ovis aries, Ctenopharyngodon idellus, Other, Chlorocebus aethiops)

IF(Mus musculus, Sus scrofa, Rattus norvegicus, Danio rerio)

IHC(Homo sapiens)

IF(Homo sapiens)

IHC(Mus musculus)

RT-PCR(Ctenopharyngodon idellus)

IP(Escherichia coli)

WB(Escherichia coli)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Caspase-3 Rabbit pAb (A0214)}

Western blot - Caspase-3 Rabbit pAb (A0214)

Western blot analysis of various lysates using Caspase-3 Rabbit pAb (A0214) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (6)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (25)