제품 > 항체 > 다중클론항체(pAb)

Caspase-8 Rabbit pAb (A0215)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Caspase-8 Rabbit pAb (A0215) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of various lysates using Caspase-8 Rabbit pAb (A0215) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Caspase-8 Rabbit pAb (A0215)

Immunohistochemistry analysis of paraffin-embedded Human colon using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat lung using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Caspase-8 Rabbit pAb (A0215)

Immunofluorescence analysis of HeLa cells using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HepG2 cells using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using Caspase-8 Rabbit pAb (A0215) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	Caspase-8 Rabbit pAb
Catalog No.	A0215
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 376-479 of human Caspase 8 (NP_203519.1).
Sequence	EEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNYEVSNKDDKKNMGKQMPQPTFTLRKKLVFPSD
Gene ID
Swiss Prot
Synonyms	CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8; Caspase-8
Calculated MW	55kDa
Observed MW	57kDa/

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	C2C12, Jurkat,
Cellular location	Cytoplasm.
Customer validation	(Eoma cell, N9) WB(Mus musculus, Bos taurus, Homo sapiens, Rattus norvegicus, Ctenopharyngodon idellus, Sus scrofa, Gallus gallus, Mus musculus, ) IHC(Rattus norvegicus) IHC(Mus musculus) RT-PCR(Ctenopharyngodon idellus)

Documents

Certificate of Compliance

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Lot number

Research Area

Caspase-8 Rabbit pAb images

Western blot - Caspase-8 Rabbit pAb (A0215)

Immunohistochemistry - Caspase-8 Rabbit pAb (A0215)

Immunofluorescence - Caspase-8 Rabbit pAb (A0215)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A0215

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Reviews List

Product Protocols

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

EMBO Molecular Medicine

Application:

IF:

12.137

Species:

Mus musculus

PMID:

29335338

Title:

CRTH2 promotes endoplasmic reticulum stress‐induced cardiomyocyte apoptosis through m‐calpain

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Frontiers in microbiology

Application:

IF:

5.64

Species:

Bos taurus

PMID:

29670605

Title:

Antiviral Immunotoxin Against Bovine herpesvirus-1: Targeted Inhibition of Viral Replication and Apoptosis of Infected Cell

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Oncology reports

Application:

IF:

3.906

Species:

Homo sapiens

PMID:

31115582

Title:

Cytokeratin 18 regulates the transcription and alternative splicing of apoptotic‑related genes and pathways in HeLa cells.

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Experimental cell research

Application:

IF:

3.905

Species:

Mus musculus

PMID:

31202710

Title:

The protective role of the MKP-5-JNK/P38 pathway in glucolipotoxicity-induced islet β-cell dysfunction and apoptosis.

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Carcinogenesis

Application:

IF:

4.944

Species:

Homo sapiens

PMID:

31306481

Title:

Histone Methyltransferase SETDB1 Promotes Colorectal Cancer Proliferation through the STAT1-CCND1/CDK6 Axis.

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

8.469

Species:

Rattus norvegicus

PMID:

31221990

Title:

Microglia-derived TNF-α mediates endothelial necroptosis aggravating blood brain-barrier disruption after ischemic stroke.

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Redox Biology

Application:

IF:

11.799

Species:

Mus musculus

PMID:

31926620

Title:

Triggering apoptosis by oroxylin A through caspase-8 activation and p62/SQSTM1 proteolysis

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Molecular Neurodegeneration

Application:

IF:

14.195

Species:

Mus musculus

PMID:

32295623

Title:

NLRP12 collaborates with NLRP3 and NLRC4 to promote pyroptosis inducing ganglion cell death of acute glaucoma

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

International Journal of Molecular Sciences

Application:

IF:

5.923

Species:

Homo sapiens

PMID:

31569691

Title:

Xanthones from the Bark of Garcinia xanthochymus and the Mechanism of Induced Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells via the Mitochondrial Pathway

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Microb Pathog

Application:

IF:

3.738

Species:

Homo sapiens

PMID:

32525021

Title:

Giardia duodenalis induces extrinsic pathway of apoptosis in intestinal epithelial cells through activation of TNFR1 and K63 de-ubiquitination of RIP1 in vitro

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Ocul Surf

Application:

IF:

5.033

Species:

Mus musculus

PMID:

32735949

Title:

NLRP12-and NLRC4-mediated corneal epithelial pyroptosis is driven by GSDMD cleavage accompanied by IL-33 processing in dry eye

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Acta Pharmacol Sin

Application:

IF:

6.15

Species:

Homo sapiens

PMID:

32747719

Title:

LW-213 induces cell apoptosis in human cutaneous T-cell lymphomas by activating PERK–eIF2α–ATF4–CHOP axis

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Food Chem Toxicol

Application:

IF:

6.023

Species:

Homo sapiens

PMID:

32619556

Title:

Glycolysis inhibition and apoptosis induction in human prostate cancer cells by FV-429-mediated regulation of AR-AKT-HK2 signaling network

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Cell Biol Toxicol

Application:

IF:

6.691

Species:

Homo sapiens

PMID:

33126266

Title:

Induction of cell cycle arrest and apoptosis by CPUC002 through stabilization of p53 and suppression of STAT3 signaling pathway in multiple myeloma

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Cell Death Dis

Application:

IF:

8.469

Species:

Homo sapiens

PMID:

33441536

Title:

FV-429 induces autophagy blockage and lysosome-dependent cell death of T-cell malignancies via lysosomal dysregulation

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

J Hazard Mater

Application:

IF:

10.588

Species:

Ctenopharyngodon idellus

PMID:

32768820

Title:

Chlorpyrifos induces the apoptosis and necroptosis of L8824 cells through the ROS/PTEN/PI3K/AKT axis

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Drug Des Devel Ther

Application:

IF:

4.16

Species:

Homo sapiens

PMID:

33833498

Title:

Chlorogenic Acid Inhibits Human Glioma U373 Cell Progression via Regulating the SRC/MAPKs Signal Pathway: Based on Network Pharmacology Analysis

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

J Hazard Mater

Application:

IF:

10.588

Species:

Sus scrofa

PMID:

34325292

Title:

Cadmium exposure triggers Oxidative stress, Necroptosis, Th1/Th2 imbalance and promotes Inflammation through the TNF-α/NF-κB pathway in swine small intestine

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Stem Cells Dev

Application:

WB, IHC

IF:

3.272

Species:

Rattus norvegicus

PMID:

34486384

Title:

Ultrasound-Guided Transplantation of Mesenchymal Stem Cells Improves Adriamycin Nephropathy in Rats through the RIPK3/MLKL and TLR-4/NF-κB signaling

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Acta Histochem

Application:

IF:

2.479

Species:

Rattus norvegicus

PMID:

34517258

Title:

Protective action of ultrasound-guided intraparenchymal transplantation of BMSCs in adriamycin nephropathy rats through the RIPK3/MLKL and NLRP3 pathways

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Bioengineered

Application:

IF:

3.269

Species:

Mus musculus

PMID:

34898368

Title:

Cell Membrane Breakage and Triggering T Cell Infiltration are Involved in Human Telomerase Reverse Transcriptase (hTERT) Promoter-driven Novel Peptide KK-64 for liver cancer gene therapy

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Frontiers in immunology

Application:

IF:

8.78

Species:

Gallus gallus

PMID:

35833119

Title:

Lnc90386 Sponges miR-33-5p to Mediate Mycoplasma gallisepticum-Induced Inflammation and Apoptosis in Chickens via the JNK Pathway - PMC

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Journal of virology

Application:

IF:

6.54

Species:

Bos taurus

PMID:

35993735

Title:

Downregulation of the Long Noncoding RNA IALNCR Targeting MAPK8/JNK1 Promotes Apoptosis and Antagonizes Bovine Viral Diarrhea Virus Replication in Host …

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Veterinary research

Application:

IF:

3.82

Species:

Homo sapiens

PMID:

35799206

Title:

Duck Tembusu virus infection induces mitochondrial-mediated and death receptor-mediated apoptosis in duck embryo fibroblasts - PMC

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

7.12

Species:

Sus scrofa

PMID:

36037632

Title:

Ammonia-induced excess ROS causes impairment and apoptosis in porcine IPEC-J2 intestinal epithelial cells

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Poultry science

Application:

IF:

4.01

Species:

Gallus gallus

PMID:

36529101

Title:

MiR-34a-5p promotes autophagy and apoptosis of ovarian granulosa cells via the Hippo-YAP signaling pathway by targeting LEF1 in chicken - PMC

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Chemosphere

Application:

IF:

8.94

Species:

Mus musculus

PMID:

35605732

Title:

Battery wastewater induces nephrotoxicity via disordering the mitochondrial dynamics

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

7.41

Species:

Homo sapiens

PMID:

36738497

Title:

Microalga Chlorella sp. extract induced apoptotic cell death of cholangiocarcinoma via AKT/mTOR signaling pathway

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Oncogene

Application:

IF:

8.75

Species:

Homo sapiens

PMID:

36352097

Title:

TNFα-induced IDH1 hyperacetylation reprograms redox homeostasis and promotes the chemotherapeutic sensitivity

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Development (Cambridge, England)

Application:

IF:

6.86

Species:

Mus musculus

PMID:

36975381

Title:

S-adenosyl-L-methionine supplementation alleviates the destructed intestinal epithelium and inflammatory infiltration caused by Mat2a deficiency

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Nutrients

Application:

IF:

6.7

Species:

Homo sapiens

PMID:

37375646

Title:

Sulforaphane Combined with Vitamin D Induces Cytotoxicity Mediated by Oxidative Stress, DNA Damage, Autophagy, and JNK/MAPK Pathway Modulation in …

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Investigational new drugs

Application:

IF:

3.651

Species:

Homo sapiens, Mus musculus

PMID:

30924024

Title:

Emodin induced necroptosis in the glioma cell line U251 via the TNF-/RIP1/RIP3 pathway.

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Food Funct

Application:

IF:

6.317

Species:

Homo sapiens

PMID:

33185634

Title:

Polycyclic polyprenylated acylphloroglucinols from Garcinia xanthochymus fruits exhibit antitumor effects by inhibition of STAT3 signaling pathway

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Frontiers in immunology

Application:

IF:

8.78

Species:

Homo sapiens

PMID:

37662906

Title:

Identification of molecular subtypes based on PANoptosis-related genes and construction of a signature for predicting the prognosis and response to immunotherapy response in hepatocellular carcinoma - PMC

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Application:

IF:

5.57

Species:

Mus musculus,

PMID:

36758785

Title:

Ganoderma lucidum extract-mediated gasdermin E cleavage promotes tumor cell pyroptosis and inhibits metastasis in breast cancer

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.89

Species:

Homo sapiens

PMID:

37154470

Title:

Echinacea Polyphenols Inhibit NLRP3-Dependent Pyroptosis, Apoptosis, and Necroptosis via Suppressing NO Production during Lipopolysaccharide-Induced Acute

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

BMC musculoskeletal disorders

Application:

IHC

IF:

2.3

Species:

Mus musculus

PMID:

38413962

Title:

Dnmt3b ablation affects fracture repair process by regulating apoptosis - PMC

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Biology

Application:

IF:

5.16

Species:

Homo sapiens

PMID:

35892954

Title:

Genistein Sensitizes Human Cholangiocarcinoma Cell Lines to Be Susceptible to Natural Killer Cells - PMC

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

The International Journal of Biochemistry & Cell Biology

Application:

IF:

5.652

Species:

Homo sapiens

PMID:

32097728

Title:

Downregulation of METTL14 increases apoptosis and autophagy induced by cisplatin in pancreatic cancer cells

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Acta Pharm Sin B

Application:

IF:

14.903

Species:

Mus musculus

PMID:

34729308

Title:

Cholesterol-associated lysosomal disorder triggers cell death of hematological malignancy: Dynamic analysis on cytotoxic effects of LW-218

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Fish & shellfish immunology

Application:

WB, RT-PCR

IF:

4.62

Species:

Ctenopharyngodon idellus

PMID:

36773714

Title:

Polysaccharide of Atractylodes macrocephala Koidz (PAMK) protects against DEHP-induced apoptosis in grass carp hepatocytes

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Frontiers in Pharmacology

Application:

IF:

5.988

Species:

Mus musculus

PMID:

29441017

Title:

15, 16-Dihydrotanshinone I Inhibits Hemangiomas through Inducing Pro-apoptotic and Anti-angiogenic Mechanisms in Vitro and in Vivo.

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Environmental toxicology

Application:

IF:

4.5

Species:

Homo sapiens

PMID:

38560766

Title:

Pachymic acid activates TP53INP2/TRAF6/caspase8 pathway to promote apoptosis in renal cell carcinoma cells

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Toxicon : official journal of the International Society on Toxinology

Application:

IF:

2.8

Species:

Homo sapiens

PMID:

38849008

Title:

Hepatoprotective effect of diammonium glycyrrhizinate and neuroprotective effect of piperazine ferulate on AmB-induced liver and kidney injury by suppressing

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Saudi Pharm J

Application:

IF:

4.1

Species:

Rattus norvegicus

PMID:

33424243

Title:

Fraxetin inhibits interleukin-1-induced apoptosis, inflammation, and matrix degradation in chondrocytes and protects rat cartilage in vivo

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Free radical biology & medicine

Application:

IF:

7.4

Species:

Homo sapiens, Mus musculus

PMID:

31560953

Title:

GL-V9 exerts anti-T cell malignancies effects via promoting lysosome-dependent AKT1 degradation and activating AKT1/FOXO3A/BIM axis.

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Cell communication and signaling : CCS

Application:

IF:

8.4

Species:

Rattus norvegicus

PMID:

39350285

Title:

Deubiquitinase USP5 regulates RIPK1 driven pyroptosis in response to myocardial ischemic reperfusion injury - PMC

References for Caspase-8 Rabbit pAb (A0215)

Product:

Caspase-8 Rabbit pAb

Journal:

Pharmaceutics

Application:

IF:

5.4

Species:

Homo sapiens

PMID:

35631550

Title:

The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death