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Caspase-9 Rabbit pAb (A2636)

Review (1)Publications (53) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Caspase-9 Rabbit pAb (A2636)

Western blot analysis of various lysates using Caspase-9 Rabbit pAb (A2636) at 1:300 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - Caspase-9 Rabbit pAb (A2636)

Western blot analysis of lysates from Jurkat cells, using Caspase-9 Rabbit pAb (A2636) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry analysis of paraffin-embedded Rat heart using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - Caspase-9 Rabbit pAb (A2636)

Immunofluorescence analysis of NIH/3T3 cells using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Caspase-9 Rabbit pAb(A2636)

Overview

Product nameCaspase-9 Rabbit pAb
Catalog No.A2636
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 139-416 of Caspase-9 (NP_001220.2).
SequenceVGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKTS
Gene ID
Swiss Prot
SynonymsMCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6; Caspase-9
Calculated MW46kDa
Observed MW36kDa/46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, K-562, Mouse liver
Cellular locationapoptosome, cytoplasm, cytosol, mitochondrion, nucleus.
Customer validation

(Human uterine stromal cells)

WB(Homo sapiens, Mus musculus, Giardia duodenalis, Holothuroidea, Sus scrofa, Rattus norvegicus, Capra hircus, Mus musculus, Other, Gallus gallus)

RT-qPCR(Homo sapiens, Mus musculus)

IHC(Mus musculus)

Documents

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ABclonal:Western blot - Caspase-9 Rabbit pAb (A2636)}

Western blot - Caspase-9 Rabbit pAb (A2636)

Western blot analysis of various lysates using Caspase-9 Rabbit pAb (A2636) at 1:300 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - Caspase-9 Rabbit pAb (A2636)}

Western blot - Caspase-9 Rabbit pAb (A2636)

Western blot analysis of lysates from Jurkat cells, using Caspase-9 Rabbit pAb (A2636) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)}

Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry analysis of paraffin-embedded Rat heart using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)}

Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)}

Immunohistochemistry - Caspase-9 Rabbit pAb (A2636)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - Caspase-9 Rabbit pAb (A2636)}

Immunofluorescence - Caspase-9 Rabbit pAb (A2636)

Immunofluorescence analysis of NIH/3T3 cells using Caspase-9 Rabbit pAb (A2636) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)