제품 > 항체 > 단일클론항체(mAb)

Cleaved PARP1 p25 Rabbit mAb (A19612)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using Cleaved PARP1 p25 Rabbit mAb (A19612) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours .
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunoprecipitation - Cleaved PARP1 p25 Rabbit mAb (A19612)

Immunoprecipitation analysis of 600 μg extracts of Jurkat cells using 3 μg Cleaved PARP1 p25 antibody (A19612). Western blot was performed from the immunoprecipitate using Cleaved PARP1 p25 antibody (A19612) at a dilution of 1:500.

Overview

Product name	Cleaved PARP1 p25 Rabbit mAb
Catalog No.	A19612
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0091

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 150-214 of human Cleaved PARP1 p25 (P09874).
Sequence	QLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVD
Gene ID
Swiss Prot
Synonyms	PARP; PARS; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; Poly-PARP; Cleaved PARP1 p25
Calculated MW	113kDa
Observed MW	27kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	Jurkat treated by Staurosporine, Mouse spleen, NIH/3T3
Cellular location	Nucleus, nucleolus
Customer validation	WB(Homo sapiens, Mus musculus, Mus musculus, ) IHC(Homo sapiens) IF(Mus musculus) IHC(Homo sapiens, Mus musculus,)

Documents

Certificate of Compliance

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Lot number

Research Area

Cleaved PARP1 p25 Rabbit mAb images

Western blot - Cleaved PARP1 p25 Rabbit mAb (A19612)

Immunoprecipitation - Cleaved PARP1 p25 Rabbit mAb (A19612)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Oncol Rep

Application:

IF:

3.906

Species:

Homo sapiens

PMID:

32945513

Title:

Curcumin enhances anti-ancer efficacy of either gemcitabine or docetaxel on pancreatic cancer cells

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Food Chem Toxicol

Application:

IF:

6.023

Species:

Homo sapiens

PMID:

32619556

Title:

Glycolysis inhibition and apoptosis induction in human prostate cancer cells by FV-429-mediated regulation of AR-AKT-HK2 signaling network

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Front Oncol

Application:

IF:

6.24

Species:

Mus musculus

PMID:

34900710

Title:

Carnosic Acid Suppresses the Development of Oral Squamous Cell Carcinoma via Mitochondrial-Mediated Apoptosis

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

BMC medicine

Application:

IF:

11.15

Species:

Homo sapiens

PMID:

36258210

Title:

NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma - PMC

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Journal of medicinal chemistry

Application:

IHC

IF:

8.03

Species:

Homo sapiens

PMID:

36331508

Title:

A 1, 2, 3-Triazole Derivative of Quinazoline Exhibits Antitumor Activity by Tethering RNF168 to SQSTM1/P62

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Cancers

Application:

IF:

6.57

Species:

Homo sapiens

PMID:

36900408

Title:

Sodium New Houttuyfonate Induces Apoptosis of Breast Cancer Cells via ROS/PDK1/AKT/GSK3β Axis - PMC

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Translational oncology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

36966611

Title:

GD2 CAR-T cells in combination with Nivolumab exhibit enhanced antitumor efficacy - PMC

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Chemico-biological interactions

Application:

IF:

5.16

Species:

Mus musculus

PMID:

37257577

Title:

AKR1B1 inhibition using NARI-29-an Epalrestat analogue-alleviates Doxorubicin-induced cardiotoxicity via modulating Calcium/CaMKII/MuRF-1 axis

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

European journal of pharmacology

Application:

IF:

5.19

Species:

Homo sapiens

PMID:

37863412

Title:

Discovery of 4-((3, 4-dichlorophenyl) amino)-2-methylquinolin-6-ol derivatives as EGFR and HDAC dual inhibitors

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Journal of enzyme inhibition and medicinal chemistry

Application:

IF:

5.75

Species:

Homo sapiens

PMID:

37357764

Title:

Design, synthesis, and evaluation of novel benzoylhydrazone derivatives as Nur77 modulators with potent antitumor activity against hepatocellular carcinoma - PMC

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Homo sapiens

PMID:

38086844

Title:

Cepharanthine, a regulator of keap1-Nrf2, inhibits gastric cancer growth through oxidative stress and energy metabolism pathway - PMC

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Scientific reports

Application:

IF:

4.6

Species:

Homo sapiens,Mus musculus

PMID:

38480798

Title:

Nortriptyline hydrochloride, a potential candidate for drug repurposing, inhibits gastric cancer by inducing oxidative stress by triggering the Keap1-Nrf2

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Cell death & disease

Application:

IF:

Species:

Mus musculus

PMID:

38378757

Title:

HRD1-induced TMEM2 ubiquitination promotes ER stress-mediated apoptosis through a non-canonical pathway in intestinal ischemia/reperfusion - PMC

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

European journal of medicinal chemistry

Application:

WB, IHC

IF:

7.08

Species:

Homo sapiens, Mus musculus,

PMID:

37837671

Title:

Design, synthesis, and biological evaluation of novel 4, 4-bipyridine derivatives acting as CDK9-Cyclin T1 protein-protein interaction inhibitors against triple

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Environmental toxicology

Application:

IF:

4.5

Species:

Homo sapiens

PMID:

38560766

Title:

Pachymic acid activates TP53INP2/TRAF6/caspase8 pathway to promote apoptosis in renal cell carcinoma cells

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Annals of medicine and surgery (2012)

Application:

IF:

1.7

Species:

Homo sapiens

PMID:

38576937

Title:

Parthenolide induces gallbladder cancer cell apoptosis via MAPK signalling - PMC

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Frontiers in molecular biosciences

Application:

IF:

Species:

Homo sapiens

PMID:

38894712

Title:

p53 deficiency mediates cisplatin resistance by upregulating RRM2 and crotonylation of RRM2K283 through the downregulation of SIRT7 - PMC

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Journal of cellular and molecular medicine

Application:

IF:

5.3

Species:

Homo sapiens

PMID:

39153211

Title:

Ophiopogonin D inhibited tumour growth and metastasis of anaplastic thyroid cancer by modulating JUN/RGS4 signalling - PMC

References for Cleaved PARP1 p25 Rabbit mAb (A19612)

Product:

Cleaved PARP1 p25 Rabbit mAb

Journal:

Bioorganic chemistry

Application:

IF:

5.1

Species:

Homo sapiens

PMID:

38735265

Title:

Biological impact and therapeutic potential of a novel camptothecin derivative (FLQY2) in pancreatic cancer through inactivation of the PDK1/AKT/mTOR pathway