Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates, using Collagen I/COL1A2 Rabbit pAb (A5786) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC):Jurkat
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human placenta using Collagen I/COL1A2 Rabbit pAb (A5786) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of A-431 cells using Collagen I/COL1A2 Rabbit pAb (A5786) at dilution of 1:25. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
| Product name | Collagen I/COL1A2 Rabbit pAb |
|---|---|
| Catalog No. | A5786 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 501-600 of human Collagen I/COL1A2 (NP_000080.2). |
|---|---|
| Sequence | TGDPGKNGDKGHAGLAGARGAPGPDGNNGAQGPPGPQGVQGGKGEQGPPGPPGFQGLPGPSGPAGEVGKPGERGLHGEFGLPGPAGPRGERGPPGESGAA |
| Gene ID | |
| Swiss Prot | |
| Synonyms | OI4; EDSCV; EDSARTH2; Collagen I/COL1A2 |
| Calculated MW | 129kDa |
| Observed MW | 165kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | Saos-2 |
| Cellular location | Secreted, extracellular matrix, extracellular space. |
| Customer validation | WB(Homo sapiens, Rattus norvegicus, Mus musculus, Other) IHC(Mus musculus) IHC(Homo sapiens, Mus musculus) IF(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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