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DDB1 Rabbit pAb (A2896)

Reviews (2)Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DDB1 Rabbit pAb (A2896)

Western blot analysis of various lysates using DDB1 Rabbit pAb (A2896) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - DDB1 Rabbit pAb (A2896)

Immunohistochemistry analysis of paraffin-embedded Rat testis using DDB1 Rabbit pAb (A2896) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - DDB1 Rabbit pAb (A2896)

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using DDB1 Rabbit pAb (A2896) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - DDB1 Rabbit pAb (A2896)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using DDB1 Rabbit pAb (A2896) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - DDB1 Rabbit pAb (A2896)

Immunofluorescence analysis of HeLa cells using DDB1 Rabbit pAb (A2896) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DDB1 Rabbit pAb (A2896)

Immunofluorescence analysis of NIH-3T3 cells using DDB1 Rabbit pAb (A2896) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for DDB1 Rabbit pAb(A2896)
Review
ABclonal: review for DDB1 Rabbit pAb(A2896)

Overview

Product nameDDB1 Rabbit pAb
Catalog No.A2896
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 741-1140 of human DDB1 (NP_001914.3).
SequenceEVQDTSGGTTALRPSASTQALSSSVSSSKLFSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGETSTPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGMKREATADDLIKVVEELTRIH
Gene ID
Swiss Prot
SynonymsXPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1; WHIKERS; DDB1
Calculated MW127kDa
Observed MW127kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesBT-474, SW620, 293T, Mouse eye, Mouse testis, Mouse breast
Cellular locationCytoplasm, Nucleus.
Customer validation

(293T, 293FT)

WB(Mus musculus, Homo sapiens, Mus musculus)

IP(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - DDB1 Rabbit pAb (A2896)}

Western blot - DDB1 Rabbit pAb (A2896)

Western blot analysis of various lysates using DDB1 Rabbit pAb (A2896) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - DDB1 Rabbit pAb (A2896)}

Immunohistochemistry - DDB1 Rabbit pAb (A2896)

Immunohistochemistry analysis of paraffin-embedded Rat testis using DDB1 Rabbit pAb (A2896) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - DDB1 Rabbit pAb (A2896)}

Immunohistochemistry - DDB1 Rabbit pAb (A2896)

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using DDB1 Rabbit pAb (A2896) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - DDB1 Rabbit pAb (A2896)}

Immunohistochemistry - DDB1 Rabbit pAb (A2896)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using DDB1 Rabbit pAb (A2896) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - DDB1 Rabbit pAb (A2896)}

Immunofluorescence - DDB1 Rabbit pAb (A2896)

Immunofluorescence analysis of HeLa cells using DDB1 Rabbit pAb (A2896) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DDB1 Rabbit pAb (A2896)}

Immunofluorescence - DDB1 Rabbit pAb (A2896)

Immunofluorescence analysis of NIH-3T3 cells using DDB1 Rabbit pAb (A2896) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)