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DDC Rabbit pAb (A3828)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DDC Rabbit pAb (A3828)

Western blot analysis of various lysates using DDC Rabbit pAb (A3828) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - DDC Rabbit pAb (A3828)

Immunohistochemistry analysis of paraffin-embedded Human small intestine using DDC Rabbit pAb (A3828) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - DDC Rabbit pAb (A3828)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using DDC Rabbit pAb (A3828) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - DDC Rabbit pAb (A3828)

Immunohistochemistry analysis of paraffin-embedded Mouse stomach using DDC Rabbit pAb (A3828) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - DDC Rabbit pAb (A3828)

Immunohistochemistry analysis of paraffin-embedded Rat brain using DDC Rabbit pAb (A3828) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - DDC Rabbit pAb (A3828)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using DDC Rabbit pAb (A3828) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - DDC Rabbit pAb (A3828)

Immunofluorescence analysis of paraffin-embedded mouse kidney using DDC Rabbit pAb (A3828) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameDDC Rabbit pAb
Catalog No.A3828
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The encoded protein catalyzes the decarboxylation of L-3, 4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-137 of human DDC (NP_000781.2).
SequenceMNASEFRRRGKEMVDYMANYMEGIEGRQVYPDVEPGYLRPLIPAAAPQEPDTFEDIINDVEKIIMPGVTHWHSPYFFAYFPTASSYPAMLADMLCGAIGCIGFSWAASPACTELETVMMDWLGKMLELPKAFLNEKA
Gene ID
Swiss Prot
SynonymsAADC; DDC
Calculated MW54kDa
Observed MW37kDa/54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSH-SY5Y, Mouse liver, Mouse brain, Mouse kidney, Rat liver, Rat kidney
Cellular locationaxon, cytoplasm, cytosol, extracellular exosome, synaptic vesicle.
Customer validation

IHC(Homo sapiens)

WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - DDC Rabbit pAb (A3828)}

Western blot - DDC Rabbit pAb (A3828)

Western blot analysis of various lysates using DDC Rabbit pAb (A3828) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - DDC Rabbit pAb (A3828)}

Immunohistochemistry - DDC Rabbit pAb (A3828)

Immunohistochemistry analysis of paraffin-embedded Human small intestine using DDC Rabbit pAb (A3828) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - DDC Rabbit pAb (A3828)}

Immunohistochemistry - DDC Rabbit pAb (A3828)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using DDC Rabbit pAb (A3828) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - DDC Rabbit pAb (A3828)}

Immunohistochemistry - DDC Rabbit pAb (A3828)

Immunohistochemistry analysis of paraffin-embedded Mouse stomach using DDC Rabbit pAb (A3828) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - DDC Rabbit pAb (A3828)}

Immunohistochemistry - DDC Rabbit pAb (A3828)

Immunohistochemistry analysis of paraffin-embedded Rat brain using DDC Rabbit pAb (A3828) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - DDC Rabbit pAb (A3828)}

Immunohistochemistry - DDC Rabbit pAb (A3828)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using DDC Rabbit pAb (A3828) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - DDC Rabbit pAb (A3828)}

Immunofluorescence - DDC Rabbit pAb (A3828)

Immunofluorescence analysis of paraffin-embedded mouse kidney using DDC Rabbit pAb (A3828) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A3828? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)