제품 > 항체 > 다중클론항체(pAb)

DDIT3/CHOP Rabbit pAb (A0221)

Review (1)Publications (59) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DDIT3/CHOP Rabbit pAb (A0221)

Western blot analysis of various lysates using DDIT3/CHOP Rabbit pAb (A0221) at 1:1000 dilution. C2C12 and C6 cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - DDIT3/CHOP Rabbit pAb (A0221)

Western blot analysis of lysates from C6 cells, using DDIT3/CHOP Rabbit pAb (A0221) at 1:2000 dilution. C6 cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - DDIT3/CHOP Rabbit pAb (A0221)

Western blot analysis of lysates from C2C12 cells, using DDIT3/CHOP Rabbit pAb (A0221) at 1:1000 dilution. C2C12 cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - DDIT3/CHOP Rabbit pAb (A0221)

Western blot analysis of lysates from C6 cells, using DDIT3/CHOP Rabbit pAb (A0221) at 1:1000 dilution. C6 cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - DDIT3/CHOP Rabbit pAb (A0221)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using DDIT3/CHOP Rabbit pAb (A0221) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - DDIT3/CHOP Rabbit pAb (A0221)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using DDIT3/CHOP Rabbit pAb (A0221) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - DDIT3/CHOP Rabbit pAb (A0221)

Immunofluorescence analysis of L929 cells using DDIT3/CHOP Rabbit pAb (A0221) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DDIT3/CHOP Rabbit pAb (A0221)

Immunofluorescence analysis of C6 cells using DDIT3/CHOP Rabbit pAb (A0221) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DDIT3/CHOP Rabbit pAb (A0221)

Immunofluorescence analysis of U2OS cells using DDIT3/CHOP Rabbit pAb (A0221) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for DDIT3/CHOP Rabbit pAb(A0221)

Overview

Product nameDDIT3/CHOP Rabbit pAb
Catalog No.A0221
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-169 of human DDIT3/CHOP (NP_004074.2).
SequenceMAAESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEEESKIFTTLDPASLAWLTEEEPEPAEVTSTSQSPHSPDSSQSSLAQEEEEEDQGRTRKRKQSGHSPARAGKQRMKEKEQENERKVAQLAEENERLKQEIERLTREVEATRRALIDRMVNLHQA
Gene ID
Swiss Prot
SynonymsCHOP; CEBPZ; CHOP10; CHOP-10; GADD153; AltDDIT3; C/EBPzeta; DDIT3/CHOP
Calculated MW19kDa
Observed MW27kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesC2C12 treated by tunicamycin, C6 treated by tunicamycin
Cellular locationCytoplasm, Nucleus.
Customer validation

(RAW264.7)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus, Stichopus japonicus Selenka, Sus scrofa, Anatinae, Other, Canis lupus familiaris)

IHC(Rattus norvegicus, Mus musculus)

IHC(Mus musculus, Rattus norvegicus)

IF(Mus musculus, Homo sapiens)

IF(Rattus norvegicus, Canis lupus familiaris)

RT-PCR(Homo sapiens)

Other(Homo sapiens)

qPCR(Other)

WB(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - DDIT3/CHOP Rabbit pAb (A0221)}

Western blot - DDIT3/CHOP Rabbit pAb (A0221)

Western blot analysis of various lysates using DDIT3/CHOP Rabbit pAb (A0221) at 1:1000 dilution. C2C12 and C6 cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - DDIT3/CHOP Rabbit pAb (A0221)}

Western blot - DDIT3/CHOP Rabbit pAb (A0221)

Western blot analysis of lysates from C6 cells, using DDIT3/CHOP Rabbit pAb (A0221) at 1:2000 dilution. C6 cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - DDIT3/CHOP Rabbit pAb (A0221)}

Western blot - DDIT3/CHOP Rabbit pAb (A0221)

Western blot analysis of lysates from C2C12 cells, using DDIT3/CHOP Rabbit pAb (A0221) at 1:1000 dilution. C2C12 cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - DDIT3/CHOP Rabbit pAb (A0221)}

Western blot - DDIT3/CHOP Rabbit pAb (A0221)

Western blot analysis of lysates from C6 cells, using DDIT3/CHOP Rabbit pAb (A0221) at 1:1000 dilution. C6 cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - DDIT3/CHOP Rabbit pAb (A0221)}

Immunohistochemistry - DDIT3/CHOP Rabbit pAb (A0221)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using DDIT3/CHOP Rabbit pAb (A0221) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - DDIT3/CHOP Rabbit pAb (A0221)}

Immunohistochemistry - DDIT3/CHOP Rabbit pAb (A0221)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using DDIT3/CHOP Rabbit pAb (A0221) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - DDIT3/CHOP Rabbit pAb (A0221)}

Immunofluorescence - DDIT3/CHOP Rabbit pAb (A0221)

Immunofluorescence analysis of L929 cells using DDIT3/CHOP Rabbit pAb (A0221) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DDIT3/CHOP Rabbit pAb (A0221)}

Immunofluorescence - DDIT3/CHOP Rabbit pAb (A0221)

Immunofluorescence analysis of C6 cells using DDIT3/CHOP Rabbit pAb (A0221) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DDIT3/CHOP Rabbit pAb (A0221)}

Immunofluorescence - DDIT3/CHOP Rabbit pAb (A0221)

Immunofluorescence analysis of U2OS cells using DDIT3/CHOP Rabbit pAb (A0221) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (3)

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Secondary Antibodies (25)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb