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DDX3X/DDX3Y Rabbit mAb (A23794)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DDX3X/DDX3Y Rabbit mAb (A23794)

Western blot analysis of various lysates, using DDX3X/DDX3Y Rabbit mAb (A23794) at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - DDX3X/DDX3Y Rabbit mAb (A23794)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using DDX3X/DDX3Y Rabbit mAb (A23794) at a dilution of  1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - DDX3X/DDX3Y Rabbit mAb (A23794)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using DDX3X/DDX3Y Rabbit mAb (A23794) at a dilution of  1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - DDX3X/DDX3Y Rabbit mAb (A23794)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using DDX3X/DDX3Y Rabbit mAb (A23794) at a dilution of  1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - DDX3X/DDX3Y Rabbit mAb (A23794)

Immunofluorescence analysis of HeLa cells using DDX3X/DDX3Y Rabbit mAb (A23794) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DDX3X/DDX3Y Rabbit mAb (A23794)

Immunofluorescence analysis of PC-12 cells using DDX3X/DDX3Y Rabbit mAb (A23794) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for DDX3X/DDX3Y Rabbit mAb(A23794)

Overview

Product nameDDX3X/DDX3Y Rabbit mAb
Catalog No.A23794
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58999
The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants.The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human DDX3X/DDX3Y. (NP_001347.3).
SequenceGRSDYDGIGSRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRY
Gene ID
Swiss Prot
SynonymsDBX; DDX3; HLP2; DDX14; CAP-Rf; MRX102; MRXSSB; DBY; DDX3X/DDX3Y
Calculated MW73kDa/71kDa/32kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100000 - 1:120000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesK-562, C2C12
Cellular locationMembrane, Cytoplasm, Cell projection
Customer validation

(RAW264.7 cells)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - DDX3X/DDX3Y Rabbit mAb (A23794)}

    Western blot - DDX3X/DDX3Y Rabbit mAb (A23794)

    Western blot analysis of various lysates, using DDX3X/DDX3Y Rabbit mAb (A23794) at 1:100000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - DDX3X/DDX3Y Rabbit mAb (A23794)}

    Immunohistochemistry - DDX3X/DDX3Y Rabbit mAb (A23794)

    Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using DDX3X/DDX3Y Rabbit mAb (A23794) at a dilution of  1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - DDX3X/DDX3Y Rabbit mAb (A23794)}

    Immunohistochemistry - DDX3X/DDX3Y Rabbit mAb (A23794)

    Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using DDX3X/DDX3Y Rabbit mAb (A23794) at a dilution of  1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - DDX3X/DDX3Y Rabbit mAb (A23794)}

    Immunohistochemistry - DDX3X/DDX3Y Rabbit mAb (A23794)

    Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using DDX3X/DDX3Y Rabbit mAb (A23794) at a dilution of  1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - DDX3X/DDX3Y Rabbit mAb (A23794)}

    Immunofluorescence - DDX3X/DDX3Y Rabbit mAb (A23794)

    Immunofluorescence analysis of HeLa cells using DDX3X/DDX3Y Rabbit mAb (A23794) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - DDX3X/DDX3Y Rabbit mAb (A23794)}

    Immunofluorescence - DDX3X/DDX3Y Rabbit mAb (A23794)

    Immunofluorescence analysis of PC-12 cells using DDX3X/DDX3Y Rabbit mAb (A23794) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)