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DDX4 Rabbit pAb (A15624)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - DDX4 Rabbit pAb (A15624)

Western blot analysis of lysates from Mouse testis, using DDX4 Rabbit pAb (A15624) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - DDX4 Rabbit pAb (A15624)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using DDX4 Rabbit pAb (A15624) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - DDX4 Rabbit pAb (A15624)

Immunofluorescence analysis of paraffin-embedded rat testis using DDX4 Rabbit pAb (A15624) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DDX4 Rabbit pAb (A15624)

Immunofluorescence analysis of paraffin-embedded mouse testis using DDX4 Rabbit pAb (A15624) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DDX4 Rabbit pAb (A15624)

Immunofluorescence analysis of paraffin-embedded rat testis using DDX4 Rabbit pAb (A15624) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DDX4 Rabbit pAb (A15624)

Immunofluorescence analysis of paraffin-embedded mouse testis using DDX4 Rabbit pAb (A15624) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameDDX4 Rabbit pAb
Catalog No.A15624
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 635-724 of human DDX4 (NP_077726.1).
SequenceGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD
Gene ID
Swiss Prot
SynonymsVASA; DDX4
Calculated MW79kDa
Observed MW79kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse testis
Cellular locationCytoplasm, perinuclear region.
Customer validation

IHC(Oncorhynchus mykiss, Homo sapiens)

WB(Nile tilapia, Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - DDX4 Rabbit pAb (A15624)}

Western blot - DDX4 Rabbit pAb (A15624)

Western blot analysis of lysates from Mouse testis, using DDX4 Rabbit pAb (A15624) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - DDX4 Rabbit pAb (A15624)}

Immunohistochemistry - DDX4 Rabbit pAb (A15624)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using DDX4 Rabbit pAb (A15624) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - DDX4 Rabbit pAb (A15624)}

Immunofluorescence - DDX4 Rabbit pAb (A15624)

Immunofluorescence analysis of paraffin-embedded rat testis using DDX4 Rabbit pAb (A15624) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DDX4 Rabbit pAb (A15624)}

Immunofluorescence - DDX4 Rabbit pAb (A15624)

Immunofluorescence analysis of paraffin-embedded mouse testis using DDX4 Rabbit pAb (A15624) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DDX4 Rabbit pAb (A15624)}

Immunofluorescence - DDX4 Rabbit pAb (A15624)

Immunofluorescence analysis of paraffin-embedded rat testis using DDX4 Rabbit pAb (A15624) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DDX4 Rabbit pAb (A15624)}

Immunofluorescence - DDX4 Rabbit pAb (A15624)

Immunofluorescence analysis of paraffin-embedded mouse testis using DDX4 Rabbit pAb (A15624) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A15624? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)