제품 > 항체 > 다중클론항체(pAb)

DHX9/RNA Helicase A Rabbit pAb (A17955)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using DHX9/RNA Helicase A Rabbit pAb (A17955) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Immunofluorescence - DHX9/RNA Helicase A Rabbit pAb (A17955)

Immunofluorescence analysis of U-2 OS cells using DHX9/RNA Helicase A Rabbit pAb (A17955) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - DHX9/RNA Helicase A Rabbit pAb (A17955)

Immunoprecipitation analysis of 500 μg extracts of HeLa cells using 3 μg DHX9/RNA Helicase Aantibody (A17955). Western blot was performed from the immunoprecipitate using DHX9/RNA Helicase Aantibody (A17955) at a dilution of 1:2000.

Overview

Product name	DHX9/RNA Helicase A Rabbit pAb
Catalog No.	A17955
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 329-563 of DHX9/RNA Helicase A (NP_001348.2).
Sequence	VVPWSPPQSNWNPWTSSNIDEGPLAFATPEQISMDLKNELMYQLEQDHDLQAILQERELLPVKKFESEILEAISQNSVVIIRGATGCGKTTQVPQFILDDFIQNDRAAECNIVVTQPRRISAVSVAERVAFERGEEPGKSCGYSVRFESILPRPHASIMFCTVGVLLRKLEAGIRGISHVIVDEIHERDINTDFLLVVLRDVVQAYPEVRIVLMSATIDTSMFCEYFFNCPIIEV
Gene ID
Swiss Prot
Synonyms	LKP; RHA; DDX9; NDH2; NDHII; DHX9/RNA Helicase A
Calculated MW	141kDa
Observed MW	150kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	HeLa, Jurkat, U-251MG
Cellular location	Cytoplasm, Nucleus, nucleolus.
Customer validation	WB(Sus scrofa, Homo sapiens, Mus musculus) ELISA(Homo sapiens, Mus musculus) IF(Mus musculus ) ChIP(Mus musculus )