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DNA Polymerase gamma Rabbit mAb (A1323)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DNA Polymerase gamma Rabbit mAb (A1323)

Western blot analysis of various lysates using DNA Polymerase gamma Rabbit mAb (A1323) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - DNA Polymerase gamma Rabbit mAb (A1323)

Western blot analysis of various lysates using DNA Polymerase gamma Rabbit mAb (A1323) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product nameDNA Polymerase gamma Rabbit mAb
Catalog No.A1323
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1871
Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1140-1239 of human DNA Polymerase gamma (P54098).
SequenceLVREEDRYRAALALQITNLLTRCMFAYKLGLNDLPQSVAFFSAVDIDRCLRKEVTMDCKTPSNPTGMERRYGIPQGEALDIYQIIELTKGSLEKRSQPGP
Gene ID
Swiss Prot
SynonymsPEO; MDP1; SCAE; MIRAS; POLG1; POLGA; SANDO; MTDPS4A; MTDPS4B; DNA Polymerase gamma
Calculated MW140kDa
Observed MW140kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHT-29, 293T, Mouse testis, Mouse brain, Rat liver, Rat testis
Cellular locationMitochondrion, Mitochondrion matrix, mitochondrion nucleoid.
Customer validation

WB(Homo sapiens, Mus musculus)

IF(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - DNA Polymerase gamma Rabbit mAb (A1323)}

Western blot - DNA Polymerase gamma Rabbit mAb (A1323)

Western blot analysis of various lysates using DNA Polymerase gamma Rabbit mAb (A1323) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - DNA Polymerase gamma Rabbit mAb (A1323)}

Western blot - DNA Polymerase gamma Rabbit mAb (A1323)

Western blot analysis of various lysates using DNA Polymerase gamma Rabbit mAb (A1323) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1323? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)