제품 > 항체 > 다중클론항체(pAb)

DNMT1 Rabbit pAb (A5495)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg DNMT1 antibody (A5495). Western blot was performed from the immunoprecipitate using DNMT1 antibody (A5495) at a dilution of 1:1000.

Review

Overview

Product name	DNMT1 Rabbit pAb
Catalog No.	A5495
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 760-1109 of DNMT1 (NP_001370.1).
Sequence	GDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNK
Gene ID
Swiss Prot
Synonyms	AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI; DNMT1
Calculated MW	183kDa
Observed MW	200kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	293T, SW480
Cellular location	Nucleus.
Customer validation	(Human hepatocellular carcinoma cell line, HUVEC, SMMC7721,BEL7404, Human cells, HEK293T, BEL-7402) WB(Homo sapiens, Mus musculus, Rattus norvegicus) IF(Homo sapiens, Oryctolagus cuniculus) IF(Homo sapiens, Rattus norvegicus) IHC(Rattus norvegicus) ChIP(Homo sapiens, Mus musculus)

Documents

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Research Area

DNMT1 Rabbit pAb images

Immunoprecipitation - DNMT1 Rabbit pAb (A5495)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for DNMT1 Rabbit pAb (A5495)

Product:

DNMT1 Rabbit pAb

Journal:

International Journal Of Biochemistry & Cell Biology

Application:

IF:

3.4

Species:

Homo sapiens

PMID:

26299326

Title:

Anticancer drugs induce hypomethylation of the acetylcholinesterase promoter via a phosphorylated-p38-DNMT1-AChE pathway in apoptotic hepatocellular carcinoma cells

References for DNMT1 Rabbit pAb (A5495)

Product:

DNMT1 Rabbit pAb

Journal:

International Journal of Nanomedicine

Application:

IF:

6.6

Species:

Homo sapiens

PMID:

29403274

Title:

Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample.

References for DNMT1 Rabbit pAb (A5495)

Product:

DNMT1 Rabbit pAb

Journal:

Nature Communications

Application:

IF:

16.6

Species:

Homo sapiens

PMID:

25960197

Title:

Molecular mechanism for USP7-mediated DNMT1 stabilization by acetylation

References for DNMT1 Rabbit pAb (A5495)

Product:

DNMT1 Rabbit pAb

Journal:

Genes Genomics

Application:

IF:

1.6

Species:

Homo sapiens

PMID:

33040302

Title:

Correlation between DNA methylation and Thymic Stromal Lymphopoietin expression in asthmatic airway epithelial cells

References for DNMT1 Rabbit pAb (A5495)

Product:

DNMT1 Rabbit pAb

Journal:

Front Oncol

Application:

IF:

3.5

Species:

Mus musculus

PMID:

34858816

Title:

Disulfiram Improves the Anti-PD-1 Therapy Efficacy by Regulating PD-L1 Expression via Epigenetically Reactivation of IRF7 in Triple Negative Breast Cancer

References for DNMT1 Rabbit pAb (A5495)

Product:

DNMT1 Rabbit pAb

Journal:

Proteomics

Application:

IF:

3.4

Species:

Mus musculus

PMID:

34951099

Title:

Quantitative proteomics revealed new functions of ALKBH4

References for DNMT1 Rabbit pAb (A5495)

Product:

DNMT1 Rabbit pAb

Journal:

Oncology research

Application:

IF:

Species:

Mus musculus

PMID:

34321149

Title:

miR-142-5p Inhibits Cell Invasion and Migration by Targeting DNMT1 in Breast Cancer

References for DNMT1 Rabbit pAb (A5495)

Product:

DNMT1 Rabbit pAb

Journal:

Journal of psychiatric research

Application:

IF:

3.7

Species:

Rattus norvegicus

PMID:

35427874

Title:

Hippocampal AMPA receptors mediate the impairment of spatial learning and memory in prenatally stressed offspring rats

References for DNMT1 Rabbit pAb (A5495)

Product:

DNMT1 Rabbit pAb

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

36856728

Title:

Maternal intermittent fasting deteriorates offspring metabolism via suppression of hepatic mTORC1 signaling

References for DNMT1 Rabbit pAb (A5495)

Product:

DNMT1 Rabbit pAb

Journal:

Frontiers in genetics

Application:

IF:

3.7

Species:

Mus musculus

PMID:

37113990

Title:

Nrf2-/- regulated lung DNA demethylation and CYP2E1 DNA methylation under PM2.5 exposure - PMC

References for DNMT1 Rabbit pAb (A5495)

Product:

DNMT1 Rabbit pAb

Journal:

Biomaterials

Application:

IF:

12.8

Species:

Homo sapiens

PMID:

29182961

Title:

Matrix stiffness determines the phenotype of vascular smooth muscle cell in vitro and in vivo: Role of DNA methyltransferase 1

References for DNMT1 Rabbit pAb (A5495)

Product:

DNMT1 Rabbit pAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Homo sapiens

PMID:

37788997

Title:

PLK1 maintains DNA methylation and cell viability by regulating phosphorylation-dependent UHRF1 protein stability - PMC

References for DNMT1 Rabbit pAb (A5495)

Product:

DNMT1 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

5.6

Species:

Homo sapiens

PMID:

37629054

Title:

Insights into the Mechanism of Curaxin CBL0137 Epigenetic Activity: The Induction of DNA Demethylation and the Suppression of BET Family Proteins - PMC

References for DNMT1 Rabbit pAb (A5495)

Product:

DNMT1 Rabbit pAb

Journal:

Cell death & disease

Application:

WB, IF

IF:

8.1

Species:

Homo sapiens

PMID: