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DNMT3B Rabbit mAb (A22658)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:ChIP-seq - DNMT3B Rabbit mAb (A22658)

Chromatin immunoprecipitation was performed with 14 μg of cross-linked chromatin from A-549 cells using 5 μg of DNMT3B Rabbit mAb (A22658). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of DNMT3B across chromosome X (upper panel) and the genomic region encompassing HUWE1, a representative gene enriched in DNMT3B (lower panel).

ABclonal:ChIP-seq - DNMT3B Rabbit mAb (A22658)

Chromatin immunoprecipitation was performed with 14 μg of cross-linked chromatin from NCCIT cells using 5 μg of DNMT3B Rabbit mAb (A22658). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of DNMT3B in the representative genomic region surrounding HUWE1 gene.

ABclonal:Western blot - DNMT3B Rabbit mAb (A22658)

Western blot analysis of various lysates using DNMT3B Rabbit mAb (A22658) at 1:2000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Chromatin Immunoprecipitation - DNMT3B Rabbit mAb (A22658)

Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from NCCIT cells, using 5 μg of DNMT3B antibody (A22658) and Rabbit IgG isotype control (AC042). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.

Overview

Product nameDNMT3B Rabbit mAb
Catalog No.A22658
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC57702
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1- 410 of human DNMT3B (NP_612564.1)
SequenceMKGDTRHLNGEEDAGGREDSILVNGACSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREVSSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRETRTRSESPAVRTRNNNSVSSRERHRPSPRSTRGRQGRNHVDESPVEFPATRSLRRRATASAGTPWPSPPSSYLTIDLTDDTEDTHGTPQSSSTPYARLAQDSQQGGMESPQVEADSGDGDSSEYQDGKEFGIGDLVWGKIKGFSWWPAMVVSWKATSKRQAMSGMRWVQWFGDGKFSEVSADKLVALGLFSQHFNLATFNKLVSYRKAMYHALEKARVRAGKTFPSSPGDSLEDQLKPMLEWAHGGFKPTGIEGLKPNNTQPVVNKSKVRRAGSRKLESRKYENKTRRRTADDSATSDYCPAPKRLKTNCYNNGKDR
Gene ID
Swiss Prot
SynonymsICF; ICF1; FSHD4; M.HsaIIIB; DNMT3B
Calculated MW96kDa
Observed MW100kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 5μg antibody for 10μg-15μg of Chromatin
  • ChIP-seq 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain, Mouse testis, F9
Cellular locationNucleus.

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ABclonal:ChIP-seq - DNMT3B Rabbit mAb (A22658)}

ChIP-seq - DNMT3B Rabbit mAb (A22658)

Chromatin immunoprecipitation was performed with 14 μg of cross-linked chromatin from A-549 cells using 5 μg of DNMT3B Rabbit mAb (A22658). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of DNMT3B across chromosome X (upper panel) and the genomic region encompassing HUWE1, a representative gene enriched in DNMT3B (lower panel).
ABclonal:ChIP-seq - DNMT3B Rabbit mAb (A22658)}

ChIP-seq - DNMT3B Rabbit mAb (A22658)

Chromatin immunoprecipitation was performed with 14 μg of cross-linked chromatin from NCCIT cells using 5 μg of DNMT3B Rabbit mAb (A22658). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of DNMT3B in the representative genomic region surrounding HUWE1 gene.
ABclonal:Western blot - DNMT3B Rabbit mAb (A22658)}

Western blot - DNMT3B Rabbit mAb (A22658)

Western blot analysis of various lysates using DNMT3B Rabbit mAb (A22658) at 1:2000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Chromatin Immunoprecipitation - DNMT3B Rabbit mAb (A22658)}

Chromatin Immunoprecipitation - DNMT3B Rabbit mAb (A22658)

Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from NCCIT cells, using 5 μg of DNMT3B antibody (A22658) and Rabbit IgG isotype control (AC042). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.

* For research use only. Not for therapeutic or diagnostic purposes.

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