DNase I (deoxyribonuclease, RNase-free ) is an endonuclease that nonspecifically cleaves DNA to release di-, tri-, and oligonucleotide products with 5'phosphorylated and 3´-hydroxylated ends. The activity of DNase I depends on Ca2+ and also be activated bydivalent metal ions Mg2+, Mn2+, etc.DNase I act on various DNAs such as single and double-stranded DNA, RNA:DNA hybrids.
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