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EAAT2/SLC1A2 Rabbit pAb (A0910)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Immunofluorescence - EAAT2/SLC1A2 Rabbit pAb (A0910)

Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse brain using EAAT2/SLC1A2 Rabbit pAb (A0910) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - EAAT2/SLC1A2 Rabbit pAb (A0910)

Immunofluorescence analysis of paraffin-embedded Rat brain tissue using EAAT2/SLC1A2 Rabbit pAb (A0910) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameEAAT2/SLC1A2 Rabbit pAb
Catalog No.A0910
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Improper regulation of this gene is thought to be associated with several neurological disorders. Alternatively spliced transcript variants of this gene have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 474-574 of human EAAT2/EAAT2/SLC1A2 (NP_004162.2).
SequenceLDRMRTSVNVVGDSFGAGIVYHLSKSELDTIDSQHRVHEDIEMTKTQSIYDDMKNHRESNSNQCVYAAHNSVIVDECKVTLAANGKSADCSVEEEPWKREK
Gene ID
Swiss Prot
SynonymsGLT1; HBGT; DEE41; EAAT2; GLT-1; EIEE41; EAAT2/SLC1A2
Calculated MW62kDa
Observed MW62kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse brain
Cellular locationMembrane, Multi-pass membrane protein
Customer validation

WB(Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

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    ABclonal:Immunofluorescence - EAAT2/SLC1A2 Rabbit pAb (A0910)}

    Immunofluorescence - EAAT2/SLC1A2 Rabbit pAb (A0910)

    Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse brain using EAAT2/SLC1A2 Rabbit pAb (A0910) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - EAAT2/SLC1A2 Rabbit pAb (A0910)}

    Immunofluorescence - EAAT2/SLC1A2 Rabbit pAb (A0910)

    Immunofluorescence analysis of paraffin-embedded Rat brain tissue using EAAT2/SLC1A2 Rabbit pAb (A0910) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A0910? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)