제품 > 항체 > 다중클론항체(pAb)

E-Cadherin Rabbit pAb (A11492)

Review (1)Publications (27) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - E-Cadherin Rabbit pAb (A11492)

Western blot analysis of lysates from HCT116 cells, using E-Cadherin Rabbit pAb (A11492) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - E-Cadherin Rabbit pAb (A11492)

Western blot analysis of lysates from Mouse liver using E-Cadherin Rabbit pAb (A11492) at 1:1500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:30s.

ABclonal:Immunohistochemistry - E-Cadherin Rabbit pAb (A11492)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using E-Cadherin Rabbit pAb (A11492) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - E-Cadherin Rabbit pAb (A11492)

Immunofluorescence analysis of A-431 cells using E-Cadherin Rabbit pAb (A11492) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - E-Cadherin Rabbit pAb (A11492)

Immunofluorescence analysis of MCF7 cells using E-Cadherin Rabbit pAb (A11492) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for E-Cadherin Rabbit pAb(A11492)

Overview

Product nameE-Cadherin Rabbit pAb
Catalog No.A11492
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 373-622 of human E-Cadherin (NP_004351.1).
SequencePIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPN
Gene ID
Swiss Prot
SynonymsUVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324; E-Cadherin
Calculated MW97kDa
Observed MW125kDa/135kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHCT116, Mouse liver
Cellular locationCell junction, Cell membrane, Endosome, Golgi apparatus, Single-pass type I membrane protein, trans-Golgi network.
Customer validation

(Human lung cancer cell)

WB(Homo sapiens, Mus musculus, Mus musculus, Rattus norvegicus)

IHC(Homo sapiens, Mus musculus)

IHC(Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - E-Cadherin Rabbit pAb (A11492)}

Western blot - E-Cadherin Rabbit pAb (A11492)

Western blot analysis of lysates from HCT116 cells, using E-Cadherin Rabbit pAb (A11492) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - E-Cadherin Rabbit pAb (A11492)}

Western blot - E-Cadherin Rabbit pAb (A11492)

Western blot analysis of lysates from Mouse liver using E-Cadherin Rabbit pAb (A11492) at 1:1500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:30s.
ABclonal:Immunohistochemistry - E-Cadherin Rabbit pAb (A11492)}

Immunohistochemistry - E-Cadherin Rabbit pAb (A11492)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using E-Cadherin Rabbit pAb (A11492) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - E-Cadherin Rabbit pAb (A11492)}

Immunofluorescence - E-Cadherin Rabbit pAb (A11492)

Immunofluorescence analysis of A-431 cells using E-Cadherin Rabbit pAb (A11492) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - E-Cadherin Rabbit pAb (A11492)}

Immunofluorescence - E-Cadherin Rabbit pAb (A11492)

Immunofluorescence analysis of MCF7 cells using E-Cadherin Rabbit pAb (A11492) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (9)

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E-Cadherin Rabbit mAb

ABflo® 488 Rabbit anti-Human E-Cadherin/CD324 mAb
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ABflo® 488 Rabbit anti-Human E-Cadherin/CD324 mAb

ABflo® 647 Rabbit anti-Human E-Cadherin/CD324 mAb
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ABflo® 647 Rabbit anti-Human E-Cadherin/CD324 mAb

ABflo® 594 Rabbit anti-Human E-Cadherin/CD324 mAb
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ABflo® 594 Rabbit anti-Human E-Cadherin/CD324 mAb

ABflo® 450 Rabbit anti-Human E-Cadherin/CD324 mAb
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ABflo® 450 Rabbit anti-Human E-Cadherin/CD324 mAb

ABflo® 700 Rabbit anti-Human E-Cadherin/CD324 mAb
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ABflo® 700 Rabbit anti-Human E-Cadherin/CD324 mAb

재조합 단백질 (1)

Recombinant Human E-Cadherin/CDH1/CD324 Protein
RP01421

Tags:C-His

Expressed Host:HEK293 cells

Recombinant Human E-Cadherin/CDH1/CD324 Protein

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)