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E-Cadherin Rabbit pAb (A3044)

Reviews (3)Publications (96) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - E-Cadherin Rabbit pAb (A3044)

Western blot analysis of various lysates using E-Cadherin Rabbit pAb (A3044) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - E-Cadherin Rabbit pAb (A3044)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using E-Cadherin Rabbit pAb (A3044) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - E-Cadherin Rabbit pAb (A3044)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using E-Cadherin Rabbit pAb (A3044) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - E-Cadherin Rabbit pAb (A3044)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using E-Cadherin Rabbit pAb (A3044) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Review
ABclonal: review for E-Cadherin Rabbit pAb(A3044)
Review
ABclonal: review for E-Cadherin Rabbit pAb(A3044)
Review
ABclonal: review for E-Cadherin Rabbit pAb(A3044)

Overview

Product nameE-Cadherin Rabbit pAb
Catalog No.A3044
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 373-622 of human E-Cadherin (NP_004351.1).
SequencePIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPN
Gene ID
Swiss Prot
SynonymsUVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324; E-Cadherin
Calculated MW97kDa
Observed MW125kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesDU145, Mouse kidney
Cellular locationCell junction, Cell membrane, Endosome, Golgi apparatus, Single-pass type I membrane protein, trans-Golgi network.
Customer validation

(mouse cells, A549)

WB(Homo sapiens, Mus musculus, Mus musculus, Rattus norvegicus, Bos taurus)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

IHC(Mus musculus, Homo sapiens, Oreochromis niloticus)

IF(Homo sapiens, Mus musculus)

ELISA(Homo sapiens, Mus musculus, Rattus norvegicus)

IHC(Homo sapiens, Mus musculus)

IHC(Homo sapiens,Rattus norvegicus)

WB(Homo sapiens, Mus musculus)

qPCR(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - E-Cadherin Rabbit pAb (A3044)}

Western blot - E-Cadherin Rabbit pAb (A3044)

Western blot analysis of various lysates using E-Cadherin Rabbit pAb (A3044) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - E-Cadherin Rabbit pAb (A3044)}

Immunohistochemistry - E-Cadherin Rabbit pAb (A3044)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using E-Cadherin Rabbit pAb (A3044) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - E-Cadherin Rabbit pAb (A3044)}

Immunohistochemistry - E-Cadherin Rabbit pAb (A3044)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using E-Cadherin Rabbit pAb (A3044) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - E-Cadherin Rabbit pAb (A3044)}

Immunohistochemistry - E-Cadherin Rabbit pAb (A3044)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using E-Cadherin Rabbit pAb (A3044) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (9)

재조합 단백질 (1)

Secondary Antibodies (26)