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EDA Rabbit pAb (A2905)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - EDA Rabbit pAb (A2905)

Western blot analysis of lysates from HepG2 cells, using EDA Rabbit pAb (A2905) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - EDA Rabbit pAb (A2905)

Immunohistochemistry analysis of paraffin-embedded Human uterine cancer using EDA Rabbit pAb (A2905) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - EDA Rabbit pAb (A2905)

Immunohistochemistry analysis of paraffin-embedded Rat liver using EDA Rabbit pAb (A2905) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - EDA Rabbit pAb (A2905)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using EDA Rabbit pAb (A2905) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - EDA Rabbit pAb (A2905)

Immunofluorescence analysis of C6 cells using EDA Rabbit pAb (A2905) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - EDA Rabbit pAb (A2905)

Immunofluorescence analysis of HepG2 cells using EDA Rabbit pAb (A2905) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - EDA Rabbit pAb (A2905)

Immunofluorescence analysis of U-251MG cells using EDA Rabbit pAb (A2905) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameEDA Rabbit pAb
Catalog No.A2905
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 62-182 of human EDA (NP_001390.1).
SequenceLELRSELRRERGAESRLGGSGTPGTSGTLSSLGGLDPDSPITSHLGQPSPKQQPLEPGEAALHSDSQDGHQMALLNFFFPDEKPYSEEESRRVRRNKRSKSNEGADGPVKNKKKGKKAGPP
Gene ID
Swiss Prot
SynonymsED1; HED; EDA1; EDA2; HED1; ODT1; XHED; ECTD1; XLHED; ED1-A1; ED1-A2; EDA-A1; EDA-A2; TNLG7C; STHAGX1; EDA
Calculated MW41kDa
Observed MW41kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2
Cellular locationCell membrane, Secreted, Single-pass type II membrane protein.

Documents

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ABclonal:Western blot - EDA Rabbit pAb (A2905)}

Western blot - EDA Rabbit pAb (A2905)

Western blot analysis of lysates from HepG2 cells, using EDA Rabbit pAb (A2905) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - EDA Rabbit pAb (A2905)}

Immunohistochemistry - EDA Rabbit pAb (A2905)

Immunohistochemistry analysis of paraffin-embedded Human uterine cancer using EDA Rabbit pAb (A2905) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - EDA Rabbit pAb (A2905)}

Immunohistochemistry - EDA Rabbit pAb (A2905)

Immunohistochemistry analysis of paraffin-embedded Rat liver using EDA Rabbit pAb (A2905) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - EDA Rabbit pAb (A2905)}

Immunohistochemistry - EDA Rabbit pAb (A2905)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using EDA Rabbit pAb (A2905) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - EDA Rabbit pAb (A2905)}

Immunofluorescence - EDA Rabbit pAb (A2905)

Immunofluorescence analysis of C6 cells using EDA Rabbit pAb (A2905) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - EDA Rabbit pAb (A2905)}

Immunofluorescence - EDA Rabbit pAb (A2905)

Immunofluorescence analysis of HepG2 cells using EDA Rabbit pAb (A2905) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - EDA Rabbit pAb (A2905)}

Immunofluorescence - EDA Rabbit pAb (A2905)

Immunofluorescence analysis of U-251MG cells using EDA Rabbit pAb (A2905) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)