제품 > 항체 > 다중클론항체(pAb)

EGFR Rabbit pAb (A11351)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from A431 cells, using EGFR Rabbit pAb (A11351) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - EGFR Rabbit pAb (A11351)

Immunohistochemistry analysis of paraffin-embedded Rat liver using EGFR Rabbit pAb (A11351) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human placenta using EGFR Rabbit pAb (A11351) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse heart using EGFR Rabbit pAb (A11351) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - EGFR Rabbit pAb (A11351)

Immunofluorescence analysis of A-431 cells using EGFR Rabbit pAb (A11351) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - EGFR Rabbit pAb (A11351)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg EGFR antibody (A11351). Western blot was performed from the immunoprecipitate using EGFR antibody (A11351) at a dilution of 1:1000.

Review

Overview

Product name	EGFR Rabbit pAb
Catalog No.	A11351
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
Sequence	QGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA
Gene ID
Swiss Prot
Synonyms	ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; EGFR
Calculated MW	134kDa
Observed MW	175kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IHC-P 1:50 - 1:200 IF/ICC 1:100 - 1:500 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	A431
Cellular location	Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Customer validation	(H1975, Human glioma) WB(Mus musculus, Zika virus, Homo sapiens) IHC(Homo sapiens) IF(Homo sapiens, Mus musculus) IF(Mus musculus, Homo sapiens) IP(Homo sapiens) FC(Homo sapiens) WB(Homo sapiens) IHC(Mus musculus) IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

EGFR Rabbit pAb images

Western blot - EGFR Rabbit pAb (A11351)

Immunohistochemistry - EGFR Rabbit pAb (A11351)

Immunofluorescence - EGFR Rabbit pAb (A11351)

Immunoprecipitation - EGFR Rabbit pAb (A11351)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A11351

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Reviews List

Product Protocols

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie

Application:

IF:

6.529

Species:

Mus musculus

PMID:

31055235

Title:

Gossypol overcomes EGFR-TKIs resistance in non-small cell lung cancer cells by targeting YAP/TAZ and EGFRL858R/T790M.

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Nat Commun

Application:

IF:

14.919

Species:

Zika virus

PMID:

32753727

Title:

Chemical proteomics tracks virus entry and uncovers NCAM1 as Zika virus receptor

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Journal Of Gene Medicine

Application:

IF:

4.565

Species:

Homo sapiens

PMID:

33151000

Title:

Downregulation of lncRNA HCG11 promotes cell proliferation of oral squamous cell carcinoma through sponging miR‐455‐5p

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

J Immunoassay Immunochem

Application:

IHC

IF:

Species:

Homo sapiens

PMID:

34106820

Title:

PDL-1 expression in lung carcinoma and its correlation with clinicopathological and prognostic characteristics

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Am J Cancer Res

Application:

WB, IF

IF:

6.166

Species:

Homo sapiens

PMID:

34249431

Title:

NONO phase separation enhances DNA damage repair by accelerating nuclear EGFR-induced DNA-PK activation

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Drug Des Devel Ther

Application:

IF:

4.162

Species:

Homo sapiens

PMID:

34511884

Title:

Integrating Network Pharmacology and Experimental Verification to Explore the Mechanism of Effect of Zuojin Pills in Pancreatic Cancer Treatment

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Current cancer drug targets

Application:

IF:

2.9

Species:

Homo sapiens

PMID:

30605061

Title:

Gefitinib represses JAK-STAT signaling activated by CRTC1-MAML2 fusion in mucoepidermoid carcinoma cells

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Homo sapiens

PMID:

35814257

Title:

LY2874455 and Abemaciclib Reverse FGF3/4/19/CCND1 Amplification Mediated Gefitinib Resistance in NSCLC - PMC

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Journal of controlled release : official journal of the Controlled Release Society

Application:

IHC

IF:

11.46

Species:

Homo sapiens

PMID:

34655677

Title:

Enhanced cancer therapeutic efficiency of NO combined with siRNA by caspase-3 responsive polymers

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Frontiers in cardiovascular medicine

Application:

IF:

5.84

Species:

Mus musculus

PMID:

35783840

Title:

Exploring the Mechanism of Hawthorn Leaves Against Coronary Heart Disease Using Network Pharmacology and Molecular Docking - PMC

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

The Science of the total environment

Application:

IF:

10.75

Species:

Mus musculus

PMID:

36526177

Title:

Combined negative effects of microplastics and plasticizer DEHP: The increased release of Nets delays wound healing in mice

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

9.68

Species:

Homo sapiens

PMID:

35725558

Title:

Co-loaded lapatinib/PAB by ferritin nanoparticles eliminated ECM-detached cluster cells via modulating EGFR in triple-negative breast cancer - PMC

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Bioorganic chemistry

Application:

IF:

5.3

Species:

Homo sapiens

PMID:

37011522

Title:

Exploration of novel dihydroquinoxalinone derivatives as EGFRL858R/T790M tyrosine kinase inhibitors for the treatment of non-small-cell lung cancer

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Homo sapiens

PMID:

37301856

Title:

HDGF promotes gefitinib resistance by activating the PI3K/AKT and MEK/ERK signaling pathways in non-small cell lung cancer - PMC

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Cell reports

Application:

IF:

Species:

Homo sapiens

PMID:

37459233

Title:

Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cells

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

Species:

Homo sapiens

PMID:

38008826

Title:

Hypoxia-induced PLOD2 promotes clear cell renal cell carcinoma progression via modulating EGFR-dependent AKT pathway activation - PMC

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

iScience

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

37953954

Title:

Targeting cuproptosis by zinc pyrithione in triple-negative breast cancer - PMC

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Frontiers in oncology

Application:

IF:

5.738

Species:

Homo sapiens

PMID:

35600368

Title:

QKI-6 Suppresses Cell Proliferation, Migration, and EMT in Non-Small Cell Lung Cancer

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Drug development research

Application:

IF:

Species:

Homo sapiens

PMID:

36852868

Title:

Kaempferol suppresses glioma progression and synergistically enhances the antitumor activity of gefitinib by inhibiting the EGFR/SRC/STAT3 signaling pathway

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

International journal of molecular sciences

Application:

WB, IF

IF:

6.2

Species:

Homo sapiens

PMID:

35887373

Title:

Targeted Co-Delivery of Gefitinib and Rapamycin by Aptamer-Modified Nanoparticles Overcomes EGFR-TKI Resistance in NSCLC via Promoting Autophagy - PMC

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

International journal of molecular sciences

Application:

WB, IF

IF:

6.2

Species:

Homo sapiens

PMID:

35887373

Title:

Targeted Co-Delivery of Gefitinib and Rapamycin by Aptamer-Modified Nanoparticles Overcomes EGFR-TKI Resistance in NSCLC via Promoting Autophagy - PMC

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

17.52

Species:

Homo sapiens

PMID:

37085943

Title:

Highly Mimetic Ex Vivo LungCancer SpheroidBased Physiological Model for Clinical Precision Therapeutics

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

ACS nano

Application:

IF:

18.02

Species:

Homo sapiens

PMID:

37120837

Title:

Bioengineered Bacteriophage-Like Nanoparticles as RNAi Therapeutics to Enhance Radiotherapy against Glioblastomas

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Nature communications

Application:

IF, WB

IF:

17.69

Species:

Homo sapiens

PMID:

38253527

Title:

EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses - PMC

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Scientific reports

Application:

IF:

4.99

Species:

Homo sapiens

PMID:

38253629

Title:

Network pharmacology combined with experimental verification to explore the potential mechanism of naringenin in the treatment of cervical cancer - PMC

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

J Control Release

Application:

WB, IHC

IF:

11.467

Species:

Mus musculus

PMID:

32860924

Title:

Biological activities of siRNA-loaded lanthanum phosphate nanoparticles on colorectal cancer

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Front Pharmacol

Application:

IF:

5.988

Species:

Mus musculus

PMID:

34867383

Title:

Network Pharmacology and Molecular Docking-Based Investigation: Prunus mume Against Colorectal Cancer via Silencing RelA Expression

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Brain research

Application:

IF:

3.61

Species:

Mus musculus

PMID:

27328426

Title:

Hyperforin promotes post-stroke functional recovery through interleukin (IL) 17A-mediated angiogenesis

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

WB, IF

IF:

7.67

Species:

Mus musculus

PMID:

37107231

Title:

Selenoprotein W Ameliorates Experimental Colitis and Promotes Intestinal Epithelial Repair - PMC

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Cancer letters

Application:

IF:

9.7

Species:

Homo sapiens

PMID:

38849009

Title:

The deubiquitinase USP2a promotes tumor immunosuppression by stabilizing immune checkpoint B7-H4 in lung adenocarcinoma harboring EGFR-activating mutants

References for EGFR Rabbit pAb (A11351)

Product:

EGFR Rabbit pAb

Journal:

Marine life science & technology

Application:

IF, IHC

IF:

5.7

Species:

Homo sapiens

PMID: