제품 > 항체 > 다중클론항체(pAb)

EIF3G Rabbit pAb (A4240)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - EIF3G Rabbit pAb (A4240)

Western blot analysis of extracts of various cell lines, using EIF3G antibody (A4240) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - EIF3G Rabbit pAb (A4240)

Immunofluorescence analysis of U-2 OS cells using EIF3G Polyclonal Antibody (A4240) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameEIF3G Rabbit pAb
Catalog No.A4240
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a core subunit of the eukaryotic translation initiation factor 3 (eIF3) complex, which is required for initiation of protein translation. An N-terminal caspase cleavage product of the encoded protein may stimulate degradation of DNA. A mutation in this gene is associated with narcolepsy.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human EIF3G (NP_003746.2).
SequenceMPTGDFDSKPSWADQVEEEGEDDKCVTSELLKGIPLATGDTSPEPELLPGAPLPPPKEVINGNIKTVTEYKIDEDGKKFKIVRTFRIETRKASKAVARRKNWKKFGNSEFDPPGPNVATTTVSDDVSMTFITSKEDLNCQEEEDPMNKLKGQKIVSCRICKGDHWTTRCPYKDTLGPMQKELAEQLGLSTGEKEKLPGELEPVQATQNKTGKYVPPSLRDGASRRGESMQPNRRADDNATIRVTNLSEDTRETDLQELFRPFGSISRIYLAKDKTTGQSKGFAFISFHRREDAARAIAGVSGFGYDHLILNVEWAKPSTN
Gene ID
Swiss Prot
SynonymsEIF3S4; EIF3-P42; eIF3-p44; eIF3-delta
Calculated MW36kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSH-SY5Y, Jurkat, HeLa, NIH/3T3, HL-60, K-562, Mouse Kidney, Mouse heart
Cellular locationCytoplasm, Nucleus, perinuclear region

Documents

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ABclonal:Western blot - EIF3G Rabbit pAb (A4240)}

Western blot - EIF3G Rabbit pAb (A4240)

Western blot analysis of extracts of various cell lines, using EIF3G antibody (A4240) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - EIF3G Rabbit pAb (A4240)}

Immunofluorescence - EIF3G Rabbit pAb (A4240)

Immunofluorescence analysis of U-2 OS cells using EIF3G Polyclonal Antibody (A4240) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.