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EMR1 Rabbit pAb (A1256)

Publications (13) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - EMR1 Rabbit pAb (A1256)

Western blot analysis of lysates from Mouse lung, using EMR1 Rabbit pAb (A1256) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - EMR1 Rabbit pAb (A1256)

Immunofluorescence analysis of paraffin-embedded Mouse spleen using EMR1 Rabbit pAb (A1256) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - EMR1 Rabbit pAb (A1256)

Immunofluorescence analysis of RAW264.7 cells using EMR1 Rabbit pAb (A1256) at dilution of 1:25 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - EMR1 Rabbit pAb (A1256)

Immunofluorescence analysis of THP-1 cells using EMR1 Rabbit pAb (A1256) at dilution of 1:25 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameEMR1 Rabbit pAb
Catalog No.A1256
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 477-596 of human EMR1 (NP_001965.3).
SequenceGVAFVSFVGMESVLNERFFKDHQAPLTTSEIKLKMNSRVVGGIMTGEKKDGFSDPIIYTLENIQPKQKFERPICVSWSTDVKGGRWTSFGCVILEASETYTICSCNQMANLAVIMASGEL
Gene ID
Swiss Prot
SynonymsEMR1; TM7LN3
Calculated MW98kDa
Observed MW160kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse lung
Cellular locationCell membrane, Multi-pass membrane protein.
Customer validation

IF(Mus musculus, Rattus norvegicus, Homo sapiens)

WB(Mus musculus)

IHC(Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - EMR1 Rabbit pAb (A1256)}

Western blot - EMR1 Rabbit pAb (A1256)

Western blot analysis of lysates from Mouse lung, using EMR1 Rabbit pAb (A1256) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - EMR1 Rabbit pAb (A1256)}

Immunofluorescence - EMR1 Rabbit pAb (A1256)

Immunofluorescence analysis of paraffin-embedded Mouse spleen using EMR1 Rabbit pAb (A1256) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - EMR1 Rabbit pAb (A1256)}

Immunofluorescence - EMR1 Rabbit pAb (A1256)

Immunofluorescence analysis of RAW264.7 cells using EMR1 Rabbit pAb (A1256) at dilution of 1:25 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - EMR1 Rabbit pAb (A1256)}

Immunofluorescence - EMR1 Rabbit pAb (A1256)

Immunofluorescence analysis of THP-1 cells using EMR1 Rabbit pAb (A1256) at dilution of 1:25 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1256? Please let us know so that we can cite the reference in this datasheet.