제품 > 항체 > 단일클론항체(mAb)

ERK1/2 Rabbit mAb (A4782)

Review (1)Publications (71) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ERK1/2 Rabbit mAb (A4782)

Western blot analysis of various lysates using ERK1/2 Rabbit mAb (A4782) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - ERK1/2 Rabbit mAb (A4782)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ERK1/2 Rabbit mAb (A4782) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ERK1/2 Rabbit mAb (A4782)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ERK1/2 Rabbit mAb (A4782) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ERK1/2 Rabbit mAb (A4782)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ERK1/2 Rabbit mAb (A4782) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - ERK1/2 Rabbit mAb (A4782)

Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb (A4782, dilution 1:50)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Review
ABclonal: review for ERK1/2 Rabbit mAb(A4782)

Overview

Product nameERK1/2 Rabbit mAb
Catalog No.A4782
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0212
This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 180-360 of human ERK2 (P28482).
SequenceHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS
Gene ID
Swiss Prot
SynonymsERK; ERK-2; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK; 5594/5595; ERK1/2
Calculated MW42, 44kDa
Observed MW42kDa/44kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, PC-12, A-431, NIH/3T3, C6, Mouse brain, Rat brain
Cellular locationcaveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane.
Customer validation

(porcine testicular interstitial cells)

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Mus musculus , Mus musculus, Rattus norvegicus, Other, Oryctolagus cuniculus, Felis catus, Sus scrofa)

ELISA(Rattus norvegicus)

WB(Rattus norvegicus)

IF(Mus musculus)

IHC(Mus musculus)

IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - ERK1/2 Rabbit mAb (A4782)}

    Western blot - ERK1/2 Rabbit mAb (A4782)

    Western blot analysis of various lysates using ERK1/2 Rabbit mAb (A4782) at 1:1000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Immunohistochemistry - ERK1/2 Rabbit mAb (A4782)}

    Immunohistochemistry - ERK1/2 Rabbit mAb (A4782)

    Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ERK1/2 Rabbit mAb (A4782) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - ERK1/2 Rabbit mAb (A4782)}

    Immunohistochemistry - ERK1/2 Rabbit mAb (A4782)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ERK1/2 Rabbit mAb (A4782) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - ERK1/2 Rabbit mAb (A4782)}

    Immunohistochemistry - ERK1/2 Rabbit mAb (A4782)

    Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ERK1/2 Rabbit mAb (A4782) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - ERK1/2 Rabbit mAb (A4782)}

    Immunofluorescence - ERK1/2 Rabbit mAb (A4782)

    Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb (A4782, dilution 1:50)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Submit an review for A4782

    Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

    Reviews List

    Publishing research using A4782? Please let us know so that we can cite the reference in this datasheet.

    항체 (7)

    Secondary Antibodies (26)