제품 > 항체 > 단일클론항체(mAb)

ERK1 / ERK2 Mouse mAb (A10613)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using ERK1 / ERK2 Mouse mAb (A10613) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Review

Overview

Product name	ERK1 / ERK2 Mouse mAb
Catalog No.	A10613
Host species	Mouse
Purification method	Affinity purification
Isotype	IgG
CloneNo.	AMC0488

Background

MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.

Immunogen information

Immunogen	A synthetic peptide of human ERK1 / ERK2
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW	36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW	42kDa/44kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa, 293T, NIH/3T3, RAW 264.7, C6, PC12
Cellular location	caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane.
Customer validation	(mouse cells) WB(Homo sapiens, Mus musculus, Rattus norvegicus, Mus musculus) IF(Mus musculus) IP(Homo sapiens) IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

ERK1 / ERK2 Mouse mAb images

Western blot - ERK1 / ERK2 Mouse mAb (A10613)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A10613

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Reviews List

Product Protocols

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Oxidative Medicine And Cellular Longevity

Application:

IF:

6.543

Species:

Homo sapiens

PMID:

28690763

Title:

Tanshinone IIA Inhibits Glutamate-Induced Oxidative Toxicity through Prevention of Mitochondrial Dysfunction and Suppression of MAPK Activation in SH-SY5Y Human Neuroblastoma Cells

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Biochemical and Biophysical Research Communications

Application:

IF:

3.575

Species:

Homo sapiens

PMID:

29412164

Title:

SGK1 inhibits PM2. 5-induced apoptosis and oxidative stress in human lung alveolar epithelial A549 cells

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Journal of experimental & clinical cancer research

Application:

IF:

11.161

Species:

Mus musculus

PMID:

30841931

Title:

Protein kinase Ds promote tumor angiogenesis through mast cell recruitment and expression of angiogenic factors in prostate cancer microenvironment.

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Cellular & molecular immunology

Application:

IF:

11.53

Species:

Mus musculus

PMID:

31467414

Title:

Essential roles of S100A10 in Toll-like receptor signaling and immunity to infection.

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Cellular & molecular immunology

Application:

IF:

11.53

Species:

Mus musculus

PMID:

1467414

Title:

Essential roles of S100A10 in Toll-like receptor signaling and immunity to infection.

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

International Journal of Biological Sciences

Application:

IF:

6.58

Species:

Rattus norvegicus

PMID:

31754334

Title:

MiR-93 regulates vascular smooth muscle cell proliferation, and neointimal formation through targeting Mfn2

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Cell Metabolism

Application:

IF:

27.287

Species:

Mus musculus

PMID:

31668872

Title:

Adipocyte Hypoxia-Inducible Factor 2α Suppresses Atherosclerosis by Promoting Adipose Ceramide Catabolism

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Frontiers in Molecular Neuroscience

Application:

IF:

5.639

Species:

Rattus norvegicus

PMID:

32116549

Title:

Lysophosphatidic Acid Induces Apoptosis of PC12 Cells Through LPA1 Receptor/LPA2 Receptor/MAPK Signaling Pathway

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

International Immunopharmacology

Application:

IF:

4.932

Species:

Mus musculus

PMID:

32335480

Title:

Isodeoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber Linn., inhibits pro-inflammatory mediators' production through both NF-κB and AP-1 pathways in LPS-activated macrophages

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Biochimica et biophysica acta. Molecular cell research

Application:

IF:

4.739

Species:

Mus musculus

PMID:

32504661

Title:

Amlexanox exerts anti-inflammatory actions by targeting phosphodiesterase 4B in lipopolysaccharide-activated macrophages

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Eur J Pharmacol

Application:

IF:

4.432

Species:

Mus musculus

PMID:

32589884

Title:

6H2L, a novel synthetic derivative of bifendate, induces apoptosis in hepatoma cells via mitochondrial and MAPK pathway

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Eur J Pharmacol

Application:

IF:

4.432

Species:

Mus musculus

PMID:

34118222

Title:

Sophoraflavanone M, a prenylated flavonoid from Sophora flavescens Ait., suppresses pro-inflammatory mediators through both NF-κB and JNK/AP-1 signaling pathways in LPS-primed macrophages

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Cell Death Discov

Application:

IF:

5.241

Species:

Homo sapiens

PMID:

34238921

Title:

Implication of human endogenous retrovirus W family envelope in hepatocellular carcinoma promotes MEK/ERK-mediated metastatic invasiveness and doxorubicin resistance

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Biomed Pharmacother

Application:

IF:

6.529

Species:

Mus musculus

PMID:

34678721

Title:

Potentilla discolor ameliorates LPS-induced inflammatory responses through suppressing NF-κB and AP-1 pathways

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

iScience

Application:

IF:

6.1

Species:

Mus musculus

PMID:

36185365

Title:

Mixed lineage kinase-like protein protects against Clostridium perfringens infection by enhancing NLRP3 inflammasome-extracellular traps axis - PMC

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Nature communications

Application:

IF:

17.69

Species:

Mus musculus

PMID:

36229481

Title:

The Hippo pathway links adipocyte plasticity to adipose tissue fibrosis - PMC

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Neurobiology of disease

Application:

IF:

7.04

Species:

Homo sapiens

PMID:

35462005

Title:

7,8-Dihydroxyflavone ameliorates mitochondrial impairment and motor dysfunction in the α-synuclein 1-103 transgenic mice

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Biochemical pharmacology

Application:

IF:

6.1

Species:

Rattus norvegicus

PMID:

36435201

Title:

Plasma exosomes confer hypoxic pulmonary hypertension by transferring LOX-1 cargo to trigger phenotypic switching of pulmonary artery smooth muscle cells

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Rattus norvegicus

PMID:

36881980

Title:

Dexmedetomidine attenuates hepatic ischemia-reperfusion injury-induced apoptosis via reducing oxidative stress and endoplasmic reticulum stress

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Phytotherapy research : PTR

Application:

IF:

6.38

Species:

Mus musculus

PMID:

36781177

Title:

Cornuside, by regulating the AGEs‐RAGE‐IκBα‐ERK1/2 signaling pathway, ameliorates cognitive impairment associated with brain aging

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

J Inflamm Res

Application:

IF:

4.631

Species:

Homo sapiens, Mus musculus

PMID:

34239314

Title:

Cadherin-11 Deficiency Attenuates Ang-II-Induced Atrial Fibrosis and Susceptibility to Atrial Fibrillation

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

PLoS Pathog

Application:

WB, IP

IF:

7.464

Species:

Homo sapiens

PMID:

34871326

Title:

RSK1 SUMOylation is required for KSHV lytic replication

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Mus musculus

PMID:

36982389

Title:

Mechanism Repositioning Based on Integrative Pharmacology: Anti-Inflammatory Effect of Safflower in Myocardial Ischemia-Reperfusion Injury - PMC

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Journal of immunology (Baltimore, Md. : 1950)

Application:

IF:

5.42

Species:

Mus musculus

PMID:

37459052

Title:

Epithelial TIPE1 Protein Guards against Colitis by Inhibiting TNF-Mediated Inflammation

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Pharmaceutics

Application:

IF:

6.52

Species:

Homo sapiens

PMID:

36986805

Title:

A Novel Fibromodulin Antagonist Peptide RP4 Exerts Antitumor Effects on Colorectal Cancer - PMC

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

Atherosclerosis

Application:

IF:

5.3

Species:

Homo sapiens

PMID:

38447436

Title:

Coptisine inhibits neointimal hyperplasia through attenuating Pak1/Pak2 signaling in vascular smooth muscle cells without retardation of re-endothelialization

References for ERK1 / ERK2 Mouse mAb (A10613)

Product:

ERK1 / ERK2 Mouse mAb

Journal:

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY

Application:

WB, IHC

IF:

4.644

Species:

Mus musculus

PMID: