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ERK1 / ERK2 Rabbit pAb (A16686)

Publications (70) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ERK1 / ERK2 Rabbit pAb (A16686)

Western blot analysis of various lysates using ERK1 / ERK2 Rabbit pAb (A16686) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using ERK1 / ERK2 Rabbit pAb (A16686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using ERK1 / ERK2 Rabbit pAb (A16686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)

Immunohistochemistry analysis of paraffin-embedded mouse heart using ERK1 / ERK2 Rabbit pAb (A16686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using ERK1 / ERK2 Rabbit pAb (A16686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)

Immunohistochemistry analysis of paraffin-embedded rat brain using ERK1 / ERK2 Rabbit pAb (A16686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)

Immunohistochemistry analysis of paraffin-embedded rat kidney using ERK1 / ERK2 Rabbit pAb (A16686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - ERK1 / ERK2 Rabbit pAb (A16686)

Immunofluorescence analysis of NIH/3T3 cells using ERK1 / ERK2 Rabbit pAb (A16686) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameERK1 / ERK2 Rabbit pAb
Catalog No.A16686
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK1 / ERK2 (NP_620407.1/NP_002737.2).
SequenceLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHK
Gene ID
Swiss Prot
SynonymsMAPK1/MAPK3; ERK1 / ERK2
Calculated MW36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW42kDa/44kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, C6, Mouse brain, Rat brain
Cellular locationcaveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane
Customer validation

WB(Homo sapiens, Mus musculus, Cyprinus carpio, Sus scrofa, Rattus norvegicus, Bos taurus, Gallus gallus, Mus musculus, Other)

IHC(Homo sapiens,Mus musculus)

IF(Mus musculus )

Co-IP(Homo sapiens,Mus musculus)

WB(Homo sapiens,Mus musculus)

Other(Oryctolagus cuniculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - ERK1 / ERK2 Rabbit pAb (A16686)}

    Western blot - ERK1 / ERK2 Rabbit pAb (A16686)

    Western blot analysis of various lysates using ERK1 / ERK2 Rabbit pAb (A16686) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)}

    Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)

    Immunohistochemistry analysis of paraffin-embedded human breast cancer using ERK1 / ERK2 Rabbit pAb (A16686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)}

    Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)

    Immunohistochemistry analysis of paraffin-embedded human lung cancer using ERK1 / ERK2 Rabbit pAb (A16686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)}

    Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)

    Immunohistochemistry analysis of paraffin-embedded mouse heart using ERK1 / ERK2 Rabbit pAb (A16686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)}

    Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)

    Immunohistochemistry analysis of paraffin-embedded mouse kidney using ERK1 / ERK2 Rabbit pAb (A16686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)}

    Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)

    Immunohistochemistry analysis of paraffin-embedded rat brain using ERK1 / ERK2 Rabbit pAb (A16686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)}

    Immunohistochemistry - ERK1 / ERK2 Rabbit pAb (A16686)

    Immunohistochemistry analysis of paraffin-embedded rat kidney using ERK1 / ERK2 Rabbit pAb (A16686) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - ERK1 / ERK2 Rabbit pAb (A16686)}

    Immunofluorescence - ERK1 / ERK2 Rabbit pAb (A16686)

    Immunofluorescence analysis of NIH/3T3 cells using ERK1 / ERK2 Rabbit pAb (A16686) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    항체 (7)

    Secondary Antibodies (26)