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FABP5 Rabbit mAb (A27255)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FABP5 Rabbit mAb (A27255)

Western blot analysis of various lysates using FABP5 Rabbit mAb (A27255) at 1:11000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): U-138 MG
Exposure time: 10s.

ABclonal:Immunofluorescence - FABP5 Rabbit mAb (A27255)

Confocal imaging of HeLa cells using FABP5 Rabbit mAb (A27255, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - FABP5 Rabbit mAb (A27255)

Confocal imaging of 293F cells using FABP5 Rabbit mAb (A27255, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - FABP5 Rabbit mAb (A27255)

Confocal imaging of paraffin-embedded Human skin tissue using FABP5 Rabbit mAb (A27255, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - FABP5 Rabbit mAb (A27255)

Confocal imaging of paraffin-embedded Human esophagus tissue using FABP5 Rabbit mAb (A27255, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - FABP5 Rabbit mAb (A27255)

Confocal imaging of paraffin-embedded Mouse brain tissue using FABP5 Rabbit mAb (A27255, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product nameFABP5 Rabbit mAb
Catalog No.A27255
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC72917
This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs may play roles in fatty acid uptake, transport, and metabolism. Polymorphisms in this gene are associated with type 2 diabetes. The human genome contains many pseudogenes similar to this locus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human FABP5 (NP_001435.1).
SequenceMATVQQLEGRWRLVDSKGFDEYMKELGVGIALRKMGAMAKPDCIITCDGKNLTIKTESTLKTTQFSCTLGEKFEETTADGRKTQTVCNFTDGALVQHQEWDGKESTITRKLKDGKLVVECVMNNVTCTRIYEKVE
Gene ID
Swiss Prot
SynonymsEFABP; KFABP; E-FABP; PAFABP; PA-FABP
Calculated MW15kDa
Observed MW15kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:11000 - 1:66000
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, 293F, Mouse brain, Mouse liver, Rat lung, Rat brain
Cellular locationCytoplasm.

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ABclonal:Western blot - FABP5 Rabbit mAb (A27255)}

Western blot - FABP5 Rabbit mAb (A27255)

Western blot analysis of various lysates using FABP5 Rabbit mAb (A27255) at 1:11000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): U-138 MG
Exposure time: 10s.
ABclonal:Immunofluorescence - FABP5 Rabbit mAb (A27255)}

Immunofluorescence - FABP5 Rabbit mAb (A27255)

Confocal imaging of HeLa cells using FABP5 Rabbit mAb (A27255, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - FABP5 Rabbit mAb (A27255)}

Immunofluorescence - FABP5 Rabbit mAb (A27255)

Confocal imaging of 293F cells using FABP5 Rabbit mAb (A27255, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - FABP5 Rabbit mAb (A27255)}

Immunofluorescence - FABP5 Rabbit mAb (A27255)

Confocal imaging of paraffin-embedded Human skin tissue using FABP5 Rabbit mAb (A27255, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
ABclonal:Immunofluorescence - FABP5 Rabbit mAb (A27255)}

Immunofluorescence - FABP5 Rabbit mAb (A27255)

Confocal imaging of paraffin-embedded Human esophagus tissue using FABP5 Rabbit mAb (A27255, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
ABclonal:Immunofluorescence - FABP5 Rabbit mAb (A27255)}

Immunofluorescence - FABP5 Rabbit mAb (A27255)

Confocal imaging of paraffin-embedded Mouse brain tissue using FABP5 Rabbit mAb (A27255, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

* For research use only. Not for therapeutic or diagnostic purposes.

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