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FANCD2 Rabbit pAb (A19311)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FANCD2 Rabbit pAb (A19311)

Western blot analysis of various lysates using FANCD2 Rabbit pAb (A19311) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - FANCD2 Rabbit pAb (A19311)

Immunofluorescence analysis of C6 cells using FANCD2 Rabbit pAb (A19311) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - FANCD2 Rabbit pAb (A19311)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug FANCD2 Rabbit pAb (A19311). Western blot was performed from the immunoprecipitate using FANCD2 Rabbit pAb (A19311) at a dilition of 1:1000.

Overview

Product nameFANCD2 Rabbit pAb
Catalog No.A19311
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-291 of FANCD2 (NP_001018125.1).
SequenceMVSKRRLSKSEDKESLTEDASKTRKQPLSKKTKKSHIANEVEENDSIFVKLLKISGIILKTGESQNQLAVDQIAFQKKLFQTLRRHPSYPKIIEEFVSGLESYIEDEDSFRNCLLSCERLQDEEASMGASYSKSLIKLLLGIDILQPAIIKTLFEKLPEYFFENKNSDEINIPRLIVSQLKWLDRVVDGKDLTTKIMQLISIAPENLQHDIITSLPEILGDSQHADVGKELSDLLIENTSLTVPILDVLSSLRLDPNFLLKVRQLVMDKLSSIRLEDLPVIIKFILHSVTA
Gene ID
Swiss Prot
SynonymsFA4; FAD; FACD; FAD2; FA-D2; FANCD; FANCD2
Calculated MW164kDa
Observed MW164kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesK-562, HeLa, A-549, C6, Mouse testis
Cellular locationNucleus.

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ABclonal:Western blot - FANCD2 Rabbit pAb (A19311)}

Western blot - FANCD2 Rabbit pAb (A19311)

Western blot analysis of various lysates using FANCD2 Rabbit pAb (A19311) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - FANCD2 Rabbit pAb (A19311)}

Immunofluorescence - FANCD2 Rabbit pAb (A19311)

Immunofluorescence analysis of C6 cells using FANCD2 Rabbit pAb (A19311) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - FANCD2 Rabbit pAb (A19311)}

Immunoprecipitation - FANCD2 Rabbit pAb (A19311)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug FANCD2 Rabbit pAb (A19311). Western blot was performed from the immunoprecipitate using FANCD2 Rabbit pAb (A19311) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)