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FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific (AS083)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Immunofluorescence - FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG,  Fc fragment specific (AS083)

Confocal imaging of paraffin-embedded Mouse brain using βIII-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with FITC F(ab')₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific(AS083, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG,  Fc fragment specific (AS083)

Confocal imaging of SH-SY5Y cells using βIII-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with FITC F(ab')₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific(AS083, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG,  Fc fragment specific (AS083)

Flow cytometry: Jurkat cells were stained with Rabbit IgG isotype control (AC042, 10 μg/mL, blue line) or CD8A Rabbit mAb (A0663, 10 μg/mL orange line), followed by FITC conjugated goat anti-Rabbit pAb (AS083, 1:200 dilution) staining. Non-fluorescently stained Jurkat cells were used as blank control (red line).

Overview

Product nameFITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
Catalog No.AS083
Host speciesGoat
Purification methodAffinity purification
IsotypeFluorescein conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:100 - 1:500
  • FC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunofluorescence - FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG,  Fc fragment specific (AS083)}

    Immunofluorescence - FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific (AS083)

    Confocal imaging of paraffin-embedded Mouse brain using βIII-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with FITC F(ab')₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific(AS083, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
    ABclonal:Immunofluorescence - FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG,  Fc fragment specific (AS083)}

    Immunofluorescence - FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific (AS083)

    Confocal imaging of SH-SY5Y cells using βIII-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with FITC F(ab')₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific(AS083, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Flow CytoMetry - FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG,  Fc fragment specific (AS083)}

    Flow CytoMetry - FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific (AS083)

    Flow cytometry: Jurkat cells were stained with Rabbit IgG isotype control (AC042, 10 μg/mL, blue line) or CD8A Rabbit mAb (A0663, 10 μg/mL orange line), followed by FITC conjugated goat anti-Rabbit pAb (AS083, 1:200 dilution) staining. Non-fluorescently stained Jurkat cells were used as blank control (red line).

    * For research use only. Not for therapeutic or diagnostic purposes.