Description
The FS Pro DNA Lib Prep Kit for Illumina is designed for DNA library preparation for next generation sequencing (NGS) on Illumina® platforms. This kit integrates DNA fragmentation with end repair and dA tailing into one step, and it can be directly followed by adapter ligation without purification. With greatly simplified experimental flow, the entire experiment can be completed within 2 hours. This kit is compatible with DNA samples from different species and different origins. Optimization of reagents and protocol greatly reduced rates of false positives, significantly improving sequencing yield and overall results, including with FFPE DNA samples. This kit is suitable for the construction of PCR-Free libraries (e.g., 100 ng of high-quality genomic DNA).
The components of the kit have undergone strict quality control, mainly including component pollution test, functional test verification, application scenario test and product batch stability test, etc., to minimize the residue of pathogenic bacteria and apply to the detection of pathogenic bacteria.
Product Features
- Perform fragmentation, end repair and dA-tailing with a single enzyme mix；
- Entire experiment completed within 2 hours.;
- Generate a wide range of insert sizes using vary fragmention time;
- Fewer inversions and lower chimeras;
- Strictly control the background bacteria of the reagent, suitable for pathogen detection and other scenarios.
- It has no dependence on DNA solvent, and can accurately interrupt DNA samples dissolved in DNase water, TE, EB and other solvents.
Workflow Chart
Storage
Store at -20℃
Product Components
|
|
Components |
8 RXN |
24 RXN |
96 RXN |
Fragmentation,
End Repair & dA Tailing |
|
FS Pro Buffer I |
40 μL |
120 μL |
480 μL |
|
FS Pro Enzymes II |
104 μL |
312 μL |
1248 μL |
|
Nuclease-Free water |
1 mL |
2 mL |
10 mL |
|
1X TE Buffer |
1 mL |
2 mL |
10 mL |
| Adapter |
|
FS Pro Ligation Buffer II |
160 μL |
480 μL |
1920 μL |
| Ligation |
|
Ligase Enzymes |
40 μL |
120 μL |
480 μL |
| Amplification |
|
2X PCR Mix |
200 μL |
600 μL |
2400 μL |
|
10X ILM PCR Primers |
40 μL |
120 μL |
480 μL |
|
MGI PCR Primer Mix |
40 μL |
120 μL |
480 μL |
Product Data
1. Generate a wide range of insert sizes using vary fragmention time.
Figure 1. Size of DNA sample library at different fragmentation times.The DNA sample is NA12878 human genome DNA standard, the input amount is 100 ng, and the fragmentation time at 32 ℃ is 5 min, 10 min, 15 min, 20 min, 25 min, 30 min. The constructed library uses Agilent DNA 1000 Chips for fragment size analysis.
1. Compatible with multiple solvents
Figure 2. The library size of DNA samples dissolved in different solvents. The DNA sample is NA12878 human genome DNA standard, and the input amount is 100 ng. Dissolved in 32 μ L Tris-HCl-pH 7.5, Nuclease-Free Water, 1X TE Buffer, EB (Elution Buffer), Low-EDTA TE Buffer, 32 ℃ fragmentation time 15 min. The constructed library uses Agilent DNA 1000 Chips for fragment size analysis.
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