Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using Ferritin Heavy Chain Rabbit pAb (A1144) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Ferritin Heavy Chain Rabbit pAb (A1144) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Ferritin Heavy Chain Rabbit pAb (A1144) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Ferritin Heavy Chain Rabbit pAb (A1144) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
| Product name | Ferritin Heavy Chain Rabbit pAb |
|---|---|
| Catalog No. | A1144 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human Ferritin Heavy Chain (NP_002023.2). |
|---|---|
| Sequence | MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES |
| Gene ID | |
| Swiss Prot | |
| Synonyms | FHC; FTH; HFE5; PLIF; FTHL6; PIG15; Ferritin Heavy Chain |
| Calculated MW | 21kDa |
| Observed MW | 21kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | RAW 264.7, C6 |
| Cellular location | cytoplasm, cytosol, extracellular exosome, extracellular region, nucleus. |
| Customer validation | WB(Homo sapiens, Mus musculus, Danio rerio, Gallus gallus) IHC(Homo sapiens, mus musculus, Mus musculus) IF(Gallus gallus, Homo sapiens) WB(Gallus gallus, Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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