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Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): SK-MEL-28
Exposure time: 30s.

ABclonal:Immunofluorescence - Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Immunofluorescence analysis of PC-12 cells using Fibroblast activation protein-α (FAP) Rabbit pAb (A6349) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Fibroblast activation protein-α (FAP) Rabbit pAb
Catalog No.	A6349
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 26-760 of human Fibroblast activation protein-α (FAP) (NP_004451.2).
Sequence	LRPSRVHNSEENTMRALTLKDILNGTFSYKTFFPNWISGQEYLHQSADNNIVLYNIETGQSYTILSNRTMKSVNASNYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLSNGEFVRGNELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITFNGRENKIFNGIPDWVYEEEMLATKYALWWSPNGKFLAYAEFNDTDIPVIAYSYYGDEQYPRTINIPYPKAGAKNPVVRIFIIDTTYPAYVGPQEVPVPAMIASSDYYFSWLTWVTDERVCLQWLKRVQNVSVLSICDFREDWQTWDCPKTQEHIEESRTGWAGGFFVSTPVFSYDAISYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAINIFRVTQDSLFYSSNEFEEYPGRRNIYRISIGSYPPSKKCVTCHLRKERCQYYTASFSDYAKYYALVCYGPGIPISTLHDGRTDQEIKILEENKELENALKNIQLPKEEIKKLEVDEITLWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVRSVFAVNWISYLASKEGMVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVRKFIEMGFIDEKRIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASVYTERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGLSGLSTNHLYTHMTHFLKQCFSLSD
Gene ID
Swiss Prot
Synonyms	FAPA; SIMP; DPPIV; FAPalpha; Fibroblast activation protein-α (FAP)
Calculated MW	88kDa
Observed MW	90kDa/95kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	U-87 MG, Rat uterus
Cellular location	Cell membrane, Cell projection, Cell surface, Cytoplasm, Membrane, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein, invadopodium membrane, lamellipodium membrane, ruffle membrane.
Customer validation	WB(Homo sapiens, Mus musculus, Mus musculus) IF(Homo sapiens, Mus musculus) RT-PCR(Rattus norvegicus) IF(Homo sapiens, Mus musculus) IHC(Homo sapiens) WB(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

연구암종양 바이오마커

Fibroblast activation protein-α (FAP) Rabbit pAb images

Western blot - Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Immunofluorescence - Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

Clin Transl Med

Application:

IF:

10.6

Species:

Homo sapiens

PMID:

33783993

Title:

Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

J Nucl Med

Application:

IF:

9.1

Species:

Homo sapiens

PMID:

34556528

Title:

Synthesis, preclinical evaluation, and a pilot clinical PET imaging study of 68Ga-labeled FAPI dimer

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

Oral diseases

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

34741375

Title:

Phenotype transition of fibroblasts incorporated into patient‐derived oral carcinoma organoids

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

Arthritis research & therapy

Application:

IF:

4.4

Species:

Mus musculus

PMID:

35729674

Title:

Myeloid cell-specific deletion of Capns1 prevents macrophage polarization toward the M1 phenotype and reduces interstitial lung disease in the bleomycin model of systemic sclerosis - PMC

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

Pharmacological research

Application:

IF:

9.1

Species:

Mus musculus

PMID:

36336217

Title:

Exosomal miR-625-3p Secreted by Cancer-associated Fibroblasts in Colorectal Cancer Promotes EMT and Chemotherapeutic Resistance by Blocking the CELF2 …

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

Theranostics

Application:

IF:

12.4

Species:

Mus musculus

PMID:

35910792

Title:

Co-delivery of phagocytosis checkpoint and STING agonist by a Trojan horse nanocapsule for orthotopic glioma immunotherapy - PMC

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

6.9

Species:

Homo sapiens

PMID:

35594704

Title:

Low-dose X-ray irradiation combined with FAK inhibitors improves the immune microenvironment and confers sensitivity to radiotherapy in pancreatic cancer

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

Frontiers in oncology

Application:

IF:

3.5

Species:

Homo sapiens

PMID:

36338684

Title:

Irradiation enhances the malignancy-promoting behaviors of cancer-associated fibroblasts - PMC

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

Respiratory research

Application:

RT-PCR

IF:

5.8

Species:

Rattus norvegicus

PMID:

37932744

Title:

[18F] -FAPI-42 PET/CT assessment of Progressive right ventricle fibrosis under pressure overload

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

International journal of biological sciences

Application:

WB, IF

IF:

8.2

Species:

Homo sapiens

PMID:

36439880

Title:

HNRNPK/CLCN3 axis facilitates the progression of LUAD through CAF-tumor interaction - PMC

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

Pharmacology research & perspectives

Application:

WB, IF

IF:

2.6

Species:

Homo sapiens, Mus musculus

PMID:

37548279

Title:

Experimental training in molecular pharmacology education based on drug-target interactions - PMC

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

Journal of dermatological science

Application:

IHC

IF:

3.8

Species:

Homo sapiens

PMID:

38155020

Title:

Mechanical stiffness promotes skin fibrosis through FAP-AKT signaling pathway

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

Cancer letters

Application:

IF:

9.1

Species:

Homo sapiens,Mus musculus

PMID:

38373690

Title:

STING inhibitors sensitize platinum chemotherapy in ovarian cancer by inhibiting the CGAS-STING pathway in cancer-associated fibroblasts (CAFs)

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

American journal of cancer research

Application:

IHC, WB

IF:

3.6

Species:

Homo sapiens

PMID:

38590418

Title:

Inactivation of ERK1/2 in cancer-associated hepatic stellate cells suppresses cancer-stromal interaction by regulating extracellular matrix in fibrosis - PMC

References for Fibroblast activation protein-α (FAP) Rabbit pAb (A6349)

Product:

Fibroblast activation protein-α (FAP) Rabbit pAb

Journal:

Journal of experimental & clinical cancer research : CR

Application:

IF:

Species:

Homo sapiens

PMID:

38825680

Title: