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GABARAP Rabbit pAb (A12568)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GABARAP Rabbit pAb (A12568)

Western blot analysis of various lysates using GABARAP Rabbit pAb (A12568) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - GABARAP Rabbit pAb (A12568)

Immunohistochemistry analysis of paraffin-embedded rat kidney using GABARAP Rabbit pAb (A12568) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GABARAP Rabbit pAb (A12568)

Immunohistochemistry analysis of paraffin-embedded human liver using GABARAP Rabbit pAb (A12568) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GABARAP Rabbit pAb (A12568)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using GABARAP Rabbit pAb (A12568) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameGABARAP Rabbit pAb
Catalog No.A12568
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human GABARAP (NP_009209.1).
SequenceMKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHE
Gene ID
Swiss Prot
SynonymsMM46; ATG8A; GABARAP-a; GABARAP
Calculated MW14kDa
Observed MW14kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHepG2, Jurkat, HeLa
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Golgi apparatus membrane, autophagosome, cytoskeleton

Documents

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ABclonal:Western blot - GABARAP Rabbit pAb (A12568)}

Western blot - GABARAP Rabbit pAb (A12568)

Western blot analysis of various lysates using GABARAP Rabbit pAb (A12568) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - GABARAP Rabbit pAb (A12568)}

Immunohistochemistry - GABARAP Rabbit pAb (A12568)

Immunohistochemistry analysis of paraffin-embedded rat kidney using GABARAP Rabbit pAb (A12568) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GABARAP Rabbit pAb (A12568)}

Immunohistochemistry - GABARAP Rabbit pAb (A12568)

Immunohistochemistry analysis of paraffin-embedded human liver using GABARAP Rabbit pAb (A12568) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GABARAP Rabbit pAb (A12568)}

Immunohistochemistry - GABARAP Rabbit pAb (A12568)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using GABARAP Rabbit pAb (A12568) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)