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GAPDH Mouse mAb (AC002)

Publications (690) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:GAPDH Mouse mAb
ABclonal:Western blot - GAPDH Mouse mAb (AC002)

Western blot analysis of various lysates using GAPDH Mouse mAb (AC002) at 1:10000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - GAPDH Mouse mAb (AC002)

Western blot analysis of lysates from HeLa cells using GAPDH Mouse mAb (AC002) at 1:5000-1:1280000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 20 s.

ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC002)

Immunohistochemistry analysis of paraffin-embedded Human kidney using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC002)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - GAPDH Mouse mAb (AC002)

Immunofluorescence analysis of NIH/3T3 cells using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameGAPDH Mouse mAb
Catalog No.AC002
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG2b, Kappa
CloneNo.AMC0062R
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (P04406).
SequenceMGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Gene ID
Swiss Prot
SynonymsG3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW36kDa
Observed MW36kDa/
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:50000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, HepG2, Mouse liver, Mouse spleen, Rat liver, Rat kidney, HeLa, 293T, Rat liver
Cellular locationCytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region.
Customer validation

WB(Mus musculus, Homo sapiens, Sus scrofa, Rattus norvegicus, Ctenopharyngodon idellus, Danio rerio, Gallus gallus, Fenneropenaeus chinensis, Yeast, Giardia duodenalis, Zika virus, Other, Capra hircus, Arabidopsis thaliana, Rhinopithecus, T. granosa, Carassius auratus, Ovis aries, Chlorocebus aethiops, Mesocricetus auratus, Canis lupus familiaris, Homo s, Mus musculus, Rattus norvegicus, Homo sapiens , Nicotiana tabacum L., Chlorocebus sabaeus, Sus scrofa, Oryctolagus cuniculus, Canis familiaris)

ELISA(Ctenopharyngodon idellus, Homo sapiens)

IF(Mus musculus, Homo sapiens)

Co-IP(Homo sapiens)

IP(Homo sapiens, Mus musculus)

IHC(Homo sapiens,Rattus norvegicus, Mus musculus)

Co-IP(Homo sapiens)

IF(Homo sapiens, Mus musculus, Chlorocebus sabaeus, Sus scrofa)

IP(Homo sapiens, Mus musculus)

ChIP(Homo sapiens)

DB(Homo sapiens)

qPCR(mus musculus)

IF(mus musculus)

ChIP(Rattus norvegicus)

WB(Homo sapiens, Mus musculus)

IHC(Homo sapiens)

RT-qPCR(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - GAPDH Mouse mAb (AC002)}

Western blot - GAPDH Mouse mAb (AC002)

Western blot analysis of various lysates using GAPDH Mouse mAb (AC002) at 1:10000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - GAPDH Mouse mAb (AC002)}

Western blot - GAPDH Mouse mAb (AC002)

Western blot analysis of lysates from HeLa cells using GAPDH Mouse mAb (AC002) at 1:5000-1:1280000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 20 s.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC002)}

Immunohistochemistry - GAPDH Mouse mAb (AC002)

Immunohistochemistry analysis of paraffin-embedded Human kidney using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC002)}

Immunohistochemistry - GAPDH Mouse mAb (AC002)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - GAPDH Mouse mAb (AC002)}

Immunofluorescence - GAPDH Mouse mAb (AC002)

Immunofluorescence analysis of NIH/3T3 cells using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (22)