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GAPDH Mouse mAb (High Dilution) (AC033)

Publications (251) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:GAPDH Mouse mAb (High Dilution)
ABclonal:Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of various lysates, using GAPDH Mouse mAb (High Dilution) (AC033) at 1:640000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of lysates from HeLa cells, using GAPDH Mouse mAb (High Dilution) (AC033) at 1:10000-1:2560000 different dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)

Immunohistochemistry analysis of paraffin-embedded Human kidney using GAPDH Mouse mAb (High Dilution) (AC033) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using GAPDH Mouse mAb (High Dilution) (AC033) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of PC-12 cells using GAPDH Mouse mAb (High Dilution) (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameGAPDH Mouse mAb (High Dilution)
Catalog No.AC033
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG2b, Kappa
CloneNo.AMC0062
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Note：Due to the high antibody titer, it is advisable to be diluted before use. Please dilute 5μL of the antibody solution with 45μL of PBS solution, containing 50% glycerol. The diluted antibody can be stored at -20°C.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (P04406).
SequenceMGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Gene ID
Swiss Prot
SynonymsG3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW36kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100000 - 1:660000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Antibody Dilute ProcedurePut 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, HepG2, Mouse kidney, Rat liver
Cellular locationCytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region.
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Other, Schisandra chinensis, Arabidopsis thaliana, Mesocricetus auratus, Mus musculus, Saccharomyces cerevisiae, Escherichia coli, Oryctolagus cuniculus, Danio rerio)

DB(Homo sapiens)

IF(Homo sapiens, Mus musculus)

Co-IP(Homo sapiens)

IP(Homo sapiens, Mus musculus)

IHC(Mus musculus, Rattus norvegicus)

IHC(Mus musculus)

ELISA(Mus musculus)

qPCR(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - GAPDH Mouse mAb (High Dilution) (AC033)}

Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of various lysates, using GAPDH Mouse mAb (High Dilution) (AC033) at 1:640000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - GAPDH Mouse mAb (High Dilution) (AC033)}

Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of lysates from HeLa cells, using GAPDH Mouse mAb (High Dilution) (AC033) at 1:10000-1:2560000 different dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)}

Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)

Immunohistochemistry analysis of paraffin-embedded Human kidney using GAPDH Mouse mAb (High Dilution) (AC033) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)}

Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using GAPDH Mouse mAb (High Dilution) (AC033) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)}

Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of PC-12 cells using GAPDH Mouse mAb (High Dilution) (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AC033? Please let us know so that we can cite the reference in this datasheet.

항체 (6)

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Reactivity:Human, Mouse, Rat

GAPDH Mouse mAb

GAPDH Rabbit mAb(High Dilution)
A19056

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

GAPDH Rabbit mAb(High Dilution)

GAPDH Rabbit pAb
AC027

WBIHC-PIF/ICCIP

Reactivity:Human, Mouse, Rat

GAPDH Rabbit pAb

GAPDH (Human Specific) Rabbit mAb
AC036

ELISAWBIF/ICC

Reactivity:Human

GAPDH (Human Specific) Rabbit mAb

HRP-conjugated GAPDH Mouse mAb
AC035

ELISAWB

Reactivity:Human, Mouse, Rat

HRP-conjugated GAPDH Mouse mAb

HRP-conjugated GAPDH Rabbit mAb
AC054

ELISAWB

Reactivity:Human, Mouse, Rat

HRP-conjugated GAPDH Rabbit mAb

Secondary Antibodies (22)

HRP-conjugated Goat anti-Mouse IgG (H+L)
AS003

WBDBELISA

HRP-conjugated Goat anti-Mouse IgG (H+L)

FITC-conjugated Goat anti-Mouse IgG (H+L)
AS001

IF/ICCFC

FITC-conjugated Goat anti-Mouse IgG (H+L)

Cy3-conjugated Goat anti-Mouse IgG (H+L)
AS008

IF/ICCFC

Cy3-conjugated Goat anti-Mouse IgG (H+L)

HRP-conjugated Goat anti-Mouse IgG Heavy Chain
AS064

WB

HRP-conjugated Goat anti-Mouse IgG Heavy Chain

HRP-conjugated Goat anti-Mouse IgG Light Chain
AS062

WB

HRP-conjugated Goat anti-Mouse IgG Light Chain

ABflo® 488-conjugated Goat anti-Mouse IgG (H+L)
AS037

IF/ICCFC

ABflo® 488-conjugated Goat anti-Mouse IgG (H+L)

HRP-conjugated Goat anti-Mouse IgG2a
AS065

WBELISA

HRP-conjugated Goat anti-Mouse IgG2a

HRP-conjugated Goat anti-Mouse IgG1
AS066

WBELISA

HRP-conjugated Goat anti-Mouse IgG1

ABflo® 594-conjugated Goat anti-Mouse IgG (H+L)
AS054

IF/ICCFC

ABflo® 594-conjugated Goat anti-Mouse IgG (H+L)

ABflo® 555-conjugated Goat anti-Mouse IgG (H+L)
AS057

IF/ICCFC

ABflo® 555-conjugated Goat anti-Mouse IgG (H+L)

TRITC-conjugated Goat anti-Mouse IgG (H+L)
AS026

IF/ICCFC

TRITC-conjugated Goat anti-Mouse IgG (H+L)

Goat Anti-Mouse IgG (H+L)
AS071

WBELISACUT&Tag

Goat Anti-Mouse IgG (H+L)

ABflo® 488-conjugated Goat anti-Mouse IgG (H+L)
AS076

IF/ICCFC

ABflo® 488-conjugated Goat anti-Mouse IgG (H+L)

HRP-conjugated Donkey anti-Mouse IgG (H+L)
AS033

WBIHC-P

HRP-conjugated Donkey anti-Mouse IgG (H+L)

AP-conjugated Goat anti-Mouse IgG (H+L)
AS017

WB

AP-conjugated Goat anti-Mouse IgG (H+L)

HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb
AS123

ELISA

HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb

ABflo® 647-conjugated Goat anti-Mouse IgG (H+L)
AS059

IF/ICCFC

ABflo® 647-conjugated Goat anti-Mouse IgG (H+L)

HRP conjugated Rabbit-Mouse IgM (Fc) mAb
AS094

WB

HRP conjugated Rabbit-Mouse IgM (Fc) mAb

HRP conjugated Rabbit Anti-Mouse IgG1 mAb
AS115

ELISA

HRP conjugated Rabbit Anti-Mouse IgG1 mAb

HRP conjugated Rabbit Anti-Mouse IgE (Fc) mAb
AS121

ELISA

HRP conjugated Rabbit Anti-Mouse IgE (Fc) mAb

ABflo® 405-conjugated Goat anti-Mouse IgG (H+L)
AS055

FC

ABflo® 405-conjugated Goat anti-Mouse IgG (H+L)

HRP conjugated Rabbit Anti-Mouse IgD (Fc) mAb
AS124

ELISA

HRP conjugated Rabbit Anti-Mouse IgD (Fc) mAb